Asian Sand Dust Enhances the Inflammatory Response and Mucin Gene Expression in the Middle Ear

장지원; 고윤영; 박무균; 채성원; 이선희; 송재준

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자료유형: 학술저널

저자정보: 장지원 고윤영 박무균 채성원 이선희 송재준

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제9권 제3호

발행연도: 2016.1

수록면: 198 - 205 (8page)

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Objectives. Asia sand dust (ASD) is known to cause various human diseases including respiratory infection. The aim of this study was to examine the effect of ASD on inflammatory response in human middle ear epithelial cells (HMEECs) in vitro and in vivo. Methods. Cell viability was assessed using the cell counting kit-8 assay. The mRNA levels of various genes including COX-2, TNF-α, MUC5AC, MUC5B, TP53, BAX, BCL-2, NOX4, and SOD1 were analyzed using semiquantitative real-time polymerase chain reaction. COX-2 protein levels were determined by western blot analysis. Sprague Dawley rats were used for in vivo investigations of inflammatory reactions in the middle ear epithelium as a result of ASD injection. Results. We observed dose-dependent decrease in HMEEC viability. ASD exposure significantly increased COX-2, TNF-α, MUC5AC, and MUC5B mRNA expression. Also, ASD affected the mRNA levels of apoptosis- and oxidative stress-related genes. Western blot analysis revealed a dose-dependent increase in COX-2 production. Animal studies also demonstrated an ASD-induced inflammatory response in the middle ear epithelium. Conclusion. Environmental ASD exposure can result in the development of otitis media.

#Keywords. Asian Sand Dust #Otitis Media #Cyclooxygenase 2 #MUC5AC #MUC5B

참고문헌 (21)

참고문헌 신청

yadav mukesh kumar / 2012 / In Vitro Streptococcus pneumoniae Biofilm Formation and In Vivo Middle Ear Mucosal Biofilm in a Rat Model of Acute Otitis Induced by S. pneumoniae / Clinical and Experimental Otorhinolaryngology 5(3):139~144

Ribeiro H. / 2003 / Air pollution and children’s health in São Paulo(1986-1998) / Soc Sci Med 57(11):2013~2022

Dostal M. / 2001 / Childhood morbidity and air pollution in the Teplice program / Cas Lek Cesk 140(21):658~661

Rovers MM. / 2006 / Risk factors for otitis media : an international perspective / Int J Pediatr Otorhinolaryngol 70(7):1251~1256

Heinrich J. / 2002 / Environmental surveys in the areas of Bitterfeld, Hettstedt and a comparative area in 1992-2000 / Gesundheitswesen 64(12):675~682

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