지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Objective: Human chorion membrane extracts (CME) are known to exhibit osteogenic effects when used for treating human osteoblast-like cells (MG63 cells), but the active compound in CME remains unknown. The aim of this study was to identify the presence of exosomes in CME and to determine the osteogenic effect of CME exosomes on MG63 cells. Methods: Exosomes were isolated from human placenta CME using the ExoQuick-TC solution and were characterized. The activity and deposition of alkaline phosphatase (ALP) on MG63 cells cultured with or without exosomes in osteogenic induction medium (OIM) were determined. Human amniotic membrane extracts (AME) were used as controls as they had not affected the osteogenic differentiation of MG63 cells in our previous study. Results: Transmission electron microscopy (TEM) revealed that exosomes isolated from CME and AME (CME-Exo and AME-Exo, respectively) had a cup-shaped structure. NanoSight™ particle tracking analysis (NTA) confirmed that the size of these exosomes was 100?150 nm. In vitro osteogenic experiments demonstrated that the exosomes from CME, but not those from AME, presented increased alkaline phosphatase (ALP) activity and resulted in the mineralization of MG63 cells in a dose-dependent manner. Conclusion: Exosomes were identified in CME and AME from the human placenta. Further, the exosomes from CME were found to be capable of promoting osteogenic differentiation, suggesting that exosomes are a key component of CME that stimulate the osteogenesis of human osteoblast-like cells. CME exosomes can be developed as promising therapeutic candidates for bone regeneration.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Théry C / 2018 / Minimal information for studies of extracellular vesicles 2018 (MISE V2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines / J Extracel Vesic 7(1):1535750
Yáñez-Mó M / 2015 / Biological properties of extracellular vesicles and their physiological functions / J Extracel Vesic 4(1):27066
Colombo M / 2014 / Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles / Ann Rev Cell Dev Biol 30(1):255~289
Valadi H / 2007 / Exosomemediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells / Nat Cell Biol 9(6):654~659
Fatima F / 2017 / Vesiculated long non-coding RNAs : offshore packages deciphering trans-regulation between cells, cancer progression and resistance to therapies / Non-coding RNA 3(1):10
Yáñez-Mó M / 2015 / Biological properties of extracellular vesicles and their physiological functions / J Extracel Vesic 4(1):27066
Colombo M / 2014 / Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles / Ann Rev Cell Dev Biol 30(1):255~289
Valadi H / 2007 / Exosomemediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells / Nat Cell Biol 9(6):654~659
Fatima F / 2017 / Vesiculated long non-coding RNAs : offshore packages deciphering trans-regulation between cells, cancer progression and resistance to therapies / Non-coding RNA 3(1):10
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A pharmaceutical investigation into exosomes
Journal of Pharmaceutical Investigation
2018 .11
-
Overcome the barriers of the skin: exosome therapy
생체재료학회지
2021 .09
-
Incidence and Risk Factors of Cystoid Macular Edema after Vitrectomy with Silicone Oil Tamponade for Retinal Detachment
Korean Journal of Ophthalmology
2018 .01
-
Complete Mesocolic Excision With Central Vascular Ligation in Comparison With Conventional Surgery for Patients With Colon Cancer – The Experiences at Two Centers
Annals of Coloproctology
2018 .01
-
The potential theragnostic (diagnostic+therapeutic) application of exosomes in diverse biomedical fields
The Korean Journal of Physiology & Pharmacology
2018 .03
-
Exosomes derived from feline adipose tissue mesenchymal stem cells reduce inflammation factors
한국실험동물학회 학술발표대회 논문집
2021 .07
-
항암치료 병용요법에 있어서 Exosome 기반 약물전달시스템의 적용
병원약사회지
2023 .05
-
Exosomes in Action: Unraveling Their Role in Autoimmune Diseases and Exploring Potential Therapeutic Applications
Immune Network
2024 .04
-
Introduction of overseas ultrasonic certification systems and CME
대한외과학회 학술대회 초록집
2015 .11
-
Exosomes as the source of biomarkers of metabolic diseases
Annals of Pediatirc Endocrinology & Metabolism
2016 .01
-
Disease-microenvironment modulation by bare- or engineered-exosome for rheumatoid arthritis treatment
생체재료학회지
2023 .03
-
Exosomal Communication Between the Tumor Microenvironment and Innate Immunity and Its Therapeutic Application
Immune Network
2022 .10
-
Comparison of short-term results after laparoscopic complete mesocolic excision and standard colectomy for right-sided colon cancer. Analysis of a Western center cohort
Annals of Coloproctology
2021 .01
-
A drug delivery system constructed by a fusion peptide capturing exosomes targets to titanium implants accurately resulting the enhancement of osseointegration peri-implant
생체재료학회지
2023 .03
-
Global Research Trends of Exosomes in the Central Nervous System: A Bibliometric and Visualized Analysis
Neurospine
2023 .06
-
Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Complete mesocolic excision in right hemicolectomy : comparison between hand-assisted laparoscopic and open approaches
Annals of Surgical Treatment and Research
2017 .02
-
Stem cell-derived exosomes for dentin-pulp complex regeneration: a mini-review
Restorative Dentistry and Endodontics
2023 .05
-
[시설운영 및 기타] Glaucoma-therapeutic effects of exosome-rich conditioned media from human amniotic membrane stem cells
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Exosome-Based Therapeutics and Challenges in Dentistry
대한치과재료학회 학술대회
2018 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0