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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.8
- 수록면
- 335 - 341 (7page)
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Purpose: Muscle mitochondria play a key role in regulating fatty acid and glucose metabolism. Dysfunction of muscle mitochondria is associated with metabolic diseases such as obesity and type 2 diabetes. Isorhamnetin (ISOR), also known as 3-O-methylquercetin, a quercetin metabolite, is a naturally occurring flavonoid in many plants. This study evaluated the effects of ISOR on the regulation of the mitochondrial function of C2C12 muscle cells.
Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method.
Results: ISOR at a concentration of 10 μM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner.
Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.
Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method.
Results: ISOR at a concentration of 10 μM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner.
Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.
목차
ABSTRACT
서론
연구방법
결과 및 고찰
REFERENCES
참고문헌 (14)
참고문헌 신청
Morino K / 2005 / Reduced mitochondrial density and increased IRS-1 serine phosphorylation in muscle of insulin-resistant offspring of type 2 diabetic parents / J Clin Invest 115(12):3587~3593
Coll PP / 2021 / The prevention of osteoporosis and sarcopenia in older adults / J Am Geriatr Soc 69(5):1388~1398
Marzetti E / 2013 / Mitochondrial dysfunction and sarcopenia of aging : from signaling pathways to clinical trials / Int J Biochem Cell Biol 45(10):2288~2301
Wu Z / 1999 / Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1 / Cell 98(1):115~124
Lima TI / 2019 / Essential role of the PGC-1α/PPARβ axis in Ucp3 gene induction / J Physiol 597(16):4277~4291
Coll PP / 2021 / The prevention of osteoporosis and sarcopenia in older adults / J Am Geriatr Soc 69(5):1388~1398
Marzetti E / 2013 / Mitochondrial dysfunction and sarcopenia of aging : from signaling pathways to clinical trials / Int J Biochem Cell Biol 45(10):2288~2301
Wu Z / 1999 / Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1 / Cell 98(1):115~124
Lima TI / 2019 / Essential role of the PGC-1α/PPARβ axis in Ucp3 gene induction / J Physiol 597(16):4277~4291
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