지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.10
- 수록면
- 827 - 843 (17page)
이용수
초록· 키워드
오류제보하기
BACKGROUND/OBJECTIVES: Mitochondrial DNA leakage leads to inflammatory responses via endosome activation. This study aims to evaluate whether the perennial grass water extract (Pogonatherum panicum) ameliorate mitochondrial DNA (mtDNA) leakage.
MATERIALS/METHODS: The major bioactive constituents of P. paniceum (PPW) were investigated by high-performance liquid chromatography, after which their antioxidant activities were assessed. In addition, RAW 264.7 macrophages were stimulated with lipopolysaccharide, resulting in mitochondrial damage. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to examine the gene expression and cytokines.
RESULTS: Our results showed that PPW extract-treated activated cells significantly decrease reactive oxygen species and nitric oxide levels by reducing the p22phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, TNF-α, IL-1β, PG-E2 and IFN-γ relative to the lipopolysaccharide (LPS)-activated macrophages. Furthermore, we observed that LPS enhanced the mtDNA leaked into the cytoplasm, increasing the transcription of Tlr9 and signaling both MyD88/Irf7-dependent interferon and MyD88/NF-κb p65-dependent inflammatory cytokine mRNA expression but which was alleviated in the presence of PPW extract.
CONCLUSIONS: Our data show that PPW extract has antioxidant and anti-inflammatory activities by facilitating mtDNA leakage and lowering the Tlr9 expression and signaling activation.
MATERIALS/METHODS: The major bioactive constituents of P. paniceum (PPW) were investigated by high-performance liquid chromatography, after which their antioxidant activities were assessed. In addition, RAW 264.7 macrophages were stimulated with lipopolysaccharide, resulting in mitochondrial damage. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to examine the gene expression and cytokines.
RESULTS: Our results showed that PPW extract-treated activated cells significantly decrease reactive oxygen species and nitric oxide levels by reducing the p22phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, TNF-α, IL-1β, PG-E2 and IFN-γ relative to the lipopolysaccharide (LPS)-activated macrophages. Furthermore, we observed that LPS enhanced the mtDNA leaked into the cytoplasm, increasing the transcription of Tlr9 and signaling both MyD88/Irf7-dependent interferon and MyD88/NF-κb p65-dependent inflammatory cytokine mRNA expression but which was alleviated in the presence of PPW extract.
CONCLUSIONS: Our data show that PPW extract has antioxidant and anti-inflammatory activities by facilitating mtDNA leakage and lowering the Tlr9 expression and signaling activation.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of Pogonatherum paniceum (Lamk) Hack extract on antimitochondrial DNA mediated inflammation by attenuating Tlr9 expression in LPS-induced macrophages
Nutrition Research and Practice
2023 .10
-
The early induction of suppressor of cytokine signaling 1 and the downregulation of toll-like receptors 7 and 9 induce tolerance in costimulated macrophages
Molecules and Cells
2015 .01
-
LPS Sensing Mechanism of Human Astrocytes : Evidence of Functional TLR4 Expression and Requirement of Soluble CD14
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2017 .12
-
RAW 264.7 대식세포에서 바위수염 메탄올 추출물에 의한 lipopolysaccharide 유도 염증성 및 산화적 스트레스의 억제
한국해양바이오학회지
2024 .12
-
Effects of TLR9/NF-κB on oxidative stress and inflammation in IPEC-J2 cells
Genes & Genomics
2022 .10
-
Anti-inflammatory effects of rutin in lipopolysaccharide-stimulated canine macrophage cells
Nutrition Research and Practice
2025 .02
-
Anti-inflammatory effects of rutin in lipopolysaccharide-stimulated canine macrophage cells
Nutrition Research and Practice
2025 .02
-
LPS-stimulated Macrophage Activation Affects Endothelial Dysfunction
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .03
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages
한국미생물·생명공학회지
2022 .12
-
Sonchus asper extract inhibits LPS-induced oxidative stress and pro-inflammatory cytokine production in RAW264.7 macrophages
Nutrition Research and Practice
2015 .12
-
Sonchus asper extract inhibits LPS-induced oxidative stress and pro-inflammatory cytokine production in RAW264.7 macrophages
Nutrition Research and Practice
2015 .12
-
Effect of saccharin on inflammation in 3T3-L1 adipocytes and the related mechanism
Nutrition Research and Practice
2020 .04
-
Effect of saccharin on inflammation in 3T3-L1 adipocytes and the related mechanism
Nutrition Research and Practice
2020 .04
-
Raw264.7 세포에서 맥문동 에탄올 추출물과 구기자 에탄올 추출물 혼합물의 항염증 효능
한국생활과학회지
2024 .10
-
Aloe-emodin inhibits Pam₃CSK₄-induced MAPK and NF-κB signaling through TLR2 in macrophages
Journal of Nutrition and Health
2016 .08
-
Black soybean anthocyanins attenuate inflammatory responses by suppressing reactive oxygen species production and mitogen activated protein kinases signaling in lipopolysaccharide-stimulated macrophages
Nutrition Research and Practice
2017 .10
-
Black soybean anthocyanins attenuate inflammatory responses by suppressing reactive oxygen species production and mitogen activated protein kinases signaling in lipopolysaccharide-stimulated macrophages
Nutrition Research and Practice
2017 .10
-
Anti-Inflammatory Effects of Hydroethanolic Extract from Ehretia asperula on Lipopolysaccharide-Stimulated RAW264.7 Macrophages
Journal of Microbiology and Biotechnology
2024 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-24-02-088162523