지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.9
- 수록면
- 199 - 207 (9page)
- DOI
- 10.7841/ksbbj.2020.35.3.199
이용수
이 논문의 연구 히스토리 (3)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Our previous research demonstrated the characteristics and fibrinolytic activities of NaCl- and capsaicin-resistant bacterium Bacillus amyloliquefaciens G-13, isolated from mustard leaf kimchi [1]. In this study, we extended the work to the comparison of various physicochemical factors (e.g., temperature, pH, NaCl, capsaicin, metal ions, and inhibitors) influencing the production of fibrinolytic enzyme by strain G-13. Initially, a time course of the fibrinolytic activity of strain G-13 was measured by the fibrin plate assay, and after 84 hours of incubation, the maximum fibrinolytic activity was about 3.42 times that of plasmin used as the standard. Through a fibrin zymographic assay, four bands (23, 34, 45, and 67 kDa) of fibrinolytic enzyme from strain G-13 were compared in fibrin zymographic gels. The strain could grow and produce the fibrinolytic enzyme in the presence of NaCl (1-10%) or capsaicin (0-300 ㎍/mL), respectively. The optimum pH and temperature for the enzymatic activity were 8 and 35℃. In the study of the effects of metal ions on fibrinolytic activity, K⁺, Ca<SUP>2+</SUP>, and Mg<SUP>2+</SUP> ions increased the activity and maintained it overall, but Fe<SUP>3+</SUP>, Zn<SUP>2+</SUP>, Ba<SUP>2+</SUP>, and Hg<SUP>2+</SUP> ions rapidly decreased the activity of the fibrinolytic enzyme. The enzyme was completely inhibited by phenylmethanesulfonyl fluroide (PMSF), suggesting that it was a serine protease. These results provide important clues for understanding the characteristics of fibrinolytic bacteria by confirming the effects of B. amyloliquefaciens G-13 on various physicochemical factors influencing the production of fibrinolytic enzymes through fibrin zymography.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (30)
참고문헌 신청
이현호 / 2019 / 갓김치에서 분리한 NaCl 및 캡사이신 내성 세균인 Bacillus amyloliquefaciens G-13의 혈전용해활성 / KSBB Journal 34(3):207~215
Mullertz, S. / 1987 / Fibrinolysis, general aspects, characteristic features and perspectives : International committee for fibrinolysiskabi prize lecture / Fibrinolysis 1:3~12
Harlan, J. M. / 1981 / Hemostasis, thrombosis, and thromboembolic disorders / Med. Clin. North. Am 65:855~857
Nakajima, N. / 1993 / Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus / Biosci. Biotechnol. Biochem 57:1726~1730
Kim, J. H. / 1999 / A Fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea / Biosci. Biotechnol. Biochem 63:2130~2136
Mullertz, S. / 1987 / Fibrinolysis, general aspects, characteristic features and perspectives : International committee for fibrinolysiskabi prize lecture / Fibrinolysis 1:3~12
Harlan, J. M. / 1981 / Hemostasis, thrombosis, and thromboembolic disorders / Med. Clin. North. Am 65:855~857
Nakajima, N. / 1993 / Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus / Biosci. Biotechnol. Biochem 57:1726~1730
Kim, J. H. / 1999 / A Fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea / Biosci. Biotechnol. Biochem 63:2130~2136
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Isolation of Bacillus Strains Showing Fibrinolytic Activity from Fermented Foods
한국생물공학회 학술대회
2021 .10
-
갓김치에서 분리한 NaCl 및 캡사이신 내성 세균인 Bacillus amyloliquefaciens G-13의 혈전용해활성
KSBB Journal
2019 .09
-
Purification and Characterization of a Fibrinolytic enzyme from Pleurotus ferulae
한국생물공학회 학술대회
2016 .10
-
Optimization of Medium Composition and Culture Conditions of Bacillus amyloliquefaciens DYC2-1
한국생물공학회 학술대회
2023 .04
-
Analysis of Gene Clusters Encoding Proteolytic Enzymes in Genome of Bacillus amyloliquefaciens SMB469
한국생물공학회 학술대회
2017 .10
-
Antifungal Activity and Characterization of Fengycins from Bacillus amyloliquefaciens subsp. plantarum BC32-1
한국생물공학회 학술대회
2015 .04
-
전통발효식품과 토양으로부터 분리된 혼합균주의 최적생육조건 및음식물쓰레기 분해 효과
Journal of Chitin and Chitosan
2018 .01
-
길항세균 Bacillus amyloliquefaciens KBC1009를 이용한 고추 흰비단병의 생물학적 방제
농업생명과학연구
2016 .08
-
신 균주 Bacillus amyloliquefaciens NBF11-1을 이용하여 발효한 청국장의 항산화효과
한국산학기술학회 논문지
2015 .08
-
Enzymes in Mesoporous Carbon for Sensitive/High-Powered Enzyme Electrodes with a Long Lifetime
한국생물공학회 학술대회
2017 .04
-
Rapid development of enzymes for biological process using bioinformatics
한국생물공학회 학술대회
2017 .10
-
Development of a Functional Mortar for Restraining Surface Algal Growth
Ecology and Resilient Infrastructure
2018 .06
-
Application of Sequence Co-Evolution and Structural Analysis for the Enhancing of Enzyme Activity
한국생물공학회 학술대회
2023 .10
-
Effect of light quality on production of antifungal metabolites of Bacillus amyloliquefaciens NBC241.
한국생물공학회 학술대회
2019 .04
-
Genetic Enzyme Screening System : A Method for High-Throughput Functional Screening of Novel Enzymes from Metagenomic Libraries
한국생물공학회 학술대회
2015 .10
-
Enzyme Adsorption, Precipitation and Crosslinking of Lipase on Polyaniline Nanofibers and Its Application
한국생물공학회 학술대회
2015 .04
-
Development of a Deep Learning-based Framework for the Prediction of Enzyme Commission Numbers
한국생물공학회 학술대회
2023 .04
-
Development of Selective Enzyme Immobilization Technology via Fusion of Gold Binding Peptides to Glucose Dehydrogenase for Multi-enzyme Based Bioelectronics Application
한국생물공학회 학술대회
2020 .10
-
청국장으로부터 단백질 분해효소 생산을 위한 Bacillus 균주 분리 및 효소 생산조건 최적화
공학기술논문지
2018 .01
-
Nanobiocatalytic Materials with Stabilized Enzymes into Magnetically-Separable Mesoporous Materials for Antifouling and Bioconversion
한국생물공학회 학술대회
2015 .04
이 논문의 저자 정보
이 논문과 함께 이용한 논문
갓김치에서 분리한 NaCl 및 캡사이신 내성 세균인 Bacillus amyloliquefaciens G-13의 혈전용해활성
이현호
,
석지원
,
오계헌
KSBB Journal
2019
.09
혈액 실험과 시뮬레이션을 통한 혈전의 성장 분석
함동휘
,
최지섭
,
김정현
외 3명
대한기계학회 춘추학술대회
2022
.11
젓갈의 숙성 및 저온 저장이 미생물 균수 및 균총에 미치는 영향
홍연
,
김정희
,
안병학
외 1명
한국식품과학회지
2000
.12
새우젓에 HACCP을 적용하기 위한 이화학적 특성 연구
조희숙
,
김경희
동아시아식생활학회지
2009
.06
고추냉이 추출물에 노출된 Bacillus cereus MK-11의 생리 및 세포반응
이현호
,
석지원
,
오계헌
KSBB Journal
2020
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2020-470-001290391