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학술저널
저자정보
이승제 ((재)전라북도생물산업진흥원) 김희정 (전라북도생물산업진흥원) 정은선 (전라북도생물산업진흥원) 박주용 (전라북도생물산업진흥원) 채종찬 (전북대학교) 황권택 (남부대학교) 김민선 (전라북도생물산업진흥원)
저널정보
한국키틴키토산학회 Journal of Chitin and Chitosan Journal of Chitin and Chitosan Vol.23 No.4
발행연도
2018.1
수록면
285 - 292 (8page)

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In this study, for the purpose of decomposing food waste, the strain was screened from traditional fermented food and soils. The enzyme activity (protease, amylase, cellulase, lipase) experiment was carried out using the paper disc method in 212 strains isolated from 5% NaCl media. Among them, only the strains having enzyme activity of more than 2 (soil) or more than 4 (traditional fermented food) with the halozone of enzyme activity of 15 mm or more were selected first, and microorganism identification through 16S rRNA sequencing was performed. Finally, were identified such as Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus siamensis, Bacillus licheniformis, Bacillus aquimaris, Bacillus megaterium, Bacillus koreensis, Bacillus stratoshericus, Bacillus aryabhattai, Bacillus safensis, Marinobacter hydrocarbonoclasticus. 11 species of mixed strains were confirmed that the culture time was 24 hours, the incubation temperature was 30oC and the optimum pH was 7.0. In order to confirm the degree of decomposition of standard food wastes (100 g) by treating 11 kinds of mixed strains (25%), solid content of more than 2000 μm was determined to be 103 g for the sterilized water group and 18 g for the mixed strains group. And the rest was decomposed to a size of less than 2000 μm.

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