지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제12호
- 발행연도
- 2019.1
- 수록면
- 1,931 - 1,937 (7page)
이용수
초록· 키워드
오류제보하기
The heterologous expression of the Streptomyces natural product (NP) biosynthetic gene cluster (BGC) has become an attractive strategy for the activation, titer improvement, and refactoring of valuable and cryptic NP BGCs. Previously, a Streptomyces artificial chromosomal vector system, pSBAC, was applied successfully to the precise cloning of large-sized polyketide BGCs, including immunosuppressant tautomycetin and antibiotic pikromycin, which led to stable and comparable production in several heterologous hosts. To further validate the pSBAC system as a generally applicable heterologous expression system, the daptomycin BGC of S. roseosporus was cloned and expressed heterologously in a model Streptomyces cell factory. A 65-kb daptomycin BGC, which belongs to a non-ribosomal polypeptide synthetase (NRPS) family, was cloned precisely into the pSBAC which resulted in 28.9 mg/l of daptomycin and its derivatives in S. coelicolor M511(a daptomycin non-producing heterologous host). These results suggest that a pSBAC-driven heterologous expression strategy is an ideal approach for producing low and inconsistent Streptomyces NRPS-family NPs, such as daptomycin, which are produced low and inconsistent in native host.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Clardy J / 2006 / New antibiotics from bacterial natural products / Nat. Biotechnol 24:1541~1550
장준필 / 2018 / Anti-Angiogenesis Effects Induced by Octaminomycins A and B against HUVECs / Journal of Microbiology and Biotechnology 28(8):1332~1338
Lam KS / 2007 / New aspects of natural products in drug discovery / Trends Microbiol 15:279~289
Genilloud O / 2011 / Current approaches to exploit actinomycetes as a source of novel natural products / J. Ind. Microbiol. Biotechnol 38:375~389
Muhanna M. Alshaibani / 2017 / Isolation, Purification, and Characterization of Five Active Diketopiperazine Derivatives from Endophytic Streptomyces SUK 25 with Antimicrobial and Cytotoxic Activities / Journal of Microbiology and Biotechnology 27(7):1249~1256
장준필 / 2018 / Anti-Angiogenesis Effects Induced by Octaminomycins A and B against HUVECs / Journal of Microbiology and Biotechnology 28(8):1332~1338
Lam KS / 2007 / New aspects of natural products in drug discovery / Trends Microbiol 15:279~289
Genilloud O / 2011 / Current approaches to exploit actinomycetes as a source of novel natural products / J. Ind. Microbiol. Biotechnol 38:375~389
Muhanna M. Alshaibani / 2017 / Isolation, Purification, and Characterization of Five Active Diketopiperazine Derivatives from Endophytic Streptomyces SUK 25 with Antimicrobial and Cytotoxic Activities / Journal of Microbiology and Biotechnology 27(7):1249~1256
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Synthetic Biology Tools for Novel Secondary Metabolite Discovery in Streptomyces
Journal of Microbiology and Biotechnology
2019 .01
-
Streptomyces BAC Cloning of a Large-Sized Biosynthetic Gene Cluster of NPP B1, a Potential SARS-CoV-2 RdRp Inhibitor
Journal of Microbiology and Biotechnology
2022 .07
-
Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster in Streptomyes Species
한국미생물학회 학술대회논문집
2019 .04
-
Ethylmalonyl-CoA Pathway Characterization and Engineering Through Which the Improved Heterologous Production of Polyketides was Achieved in Streptomyces venezuelae
한국미생물학회 학술대회논문집
2015 .04
-
An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
Journal of Microbiology and Biotechnology
2021 .12
-
Screening and molecular identification of Streptomyces species isolated from high altitude soil of Nepal
미생물학회지
2021 .09
-
Genetic Strategies for Pikromycin Over-production in Streptomyces venezuelae
한국미생물학회 학술대회논문집
2016 .04
-
식물 유용 방선균 2종의 배양 및 포자생성 최적화 조건 탐색
Research in Plant Disease(식물병연구)
2023 .06
-
A CRISPR/Cas9 Cleavage System for Capturing Fungal Secondary Metabolite Gene Clusters
Journal of Microbiology and Biotechnology
2021 .01
-
Molecular Mechanism of Zinc-dependent Gene Activation by Zur in Streptomyces coelicolor
한국미생물학회 학술대회논문집
2022 .10
-
Identification of a Polyketide Pathway for the Biosynthesis of Atranorin by Genome Mining and Heterologous Expression
한국미생물학회 학술대회논문집
2021 .08
-
Donor Plasmid System for Rapid and Markerless Integration of Heterologous Genes into Bacterial Chromosomes
한국미생물학회 학술대회논문집
2019 .04
-
RecET Recombineering System for Markerless Integration of Heterologous Biosynthetic Gene Clusters into Chromosomal DNA of Pseudomonas putida
한국미생물학회 학술대회논문집
2018 .04
-
Draft Genome Analysis of Antimicrobial Streptomyces Isolated from Himalayan Lichen
Journal of Microbiology and Biotechnology
2019 .01
-
RecET Recombineering System for Markerless Integration of Heterologous Biosynthetic Genes to Pseudomonas putida Chromosome
한국미생물학회 학술대회논문집
2019 .04
-
Division of Labor among Oxidative Stress Response Regulators in Streptomyces coelicolor
한국미생물학회 학술대회논문집
2019 .04
-
Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis
Journal of Microbiology and Biotechnology
2015 .01
-
Isolation and Characterization of Antimicrobial Compounds Produced from Streptomyces spp.
한국미생물학회 학술대회논문집
2015 .04
-
Effects of Sucrose, Phosphate, and Calcium Carbonate on the Production of Pikromycin from Streptomyces venezuelae
Journal of Microbiology and Biotechnology
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0