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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제4호
- 발행연도
- 2015.1
- 수록면
- 496 - 502 (7page)
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Polyketide secondary metabolites share common precursor pools, acyl-CoA. Thus, the effects of engineering strategies for heterologous and native secondary metabolite production are often determined by the measurement of pikromycin in Streptomyces venezuelae. It is hard to compare the effectiveness of engineering targets among published data owing to the different pikromycin production media used from one study to the other. To determine the most important nutritional factor and establish optimal culture conditions, medium optimization of pikromycin from Streptomyces venezuelae ATCC 15439 was studied with a statistical method, Plackett-Burman design. Nine variables (glucose, sucrose, peptone, (NH4)2SO4, K2HPO4, KH2PO4, NaCl, MgSO4·7H2O, and CaCO3) were analyzed for their effects on a response, pikromycin. Glucose, K2HPO4, and CaCO3 were determined to be the most significant factors. The path of the steepest ascent and response surface methodology about the three selected components were performed to study interactions among the three factors, and the fine-tune concentrations for maximized product yields. The significant variables and optimal concentrations were 139 g/1 sucrose, 5.29 g/l K2HPO4, and 0.081 g/l CaCO3, with the maximal pikromycin yield of 35.5 mg/l. Increases of the antibiotics production by 1.45-fold, 1.3-fold, and 1.98-fold, compared with unoptimized medium and two other pikromycin production media SCM and SGGP, respectively, were achieved.
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참고문헌 (24)
참고문헌 신청
Abbas AS / 1990 / Effects of metals on Streptomyces coelicolor growth and actinorhodin production / Appl. Environ. Microbiol 56 : 675 ~ 680
Aguirre AM / 2013 / Investigation of biomass concentration, lipid production, and cellulose content in Chlorella vulgaris cultures using response surface methodology / Biotec
Berdy J / 2005 / Bioactive microbial metabolites / J. Antibiotics 58 : 1 ~ 26
Bogs J / 2000 / Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence / J. Bacteriol 182 : 5351 ~ 5358
Burke J / 2001 / Generalized transduction in Streptomyces coelicolor / Proc. Nat. Acad. Sci. USA 98 : 6289 ~ 6294
Aguirre AM / 2013 / Investigation of biomass concentration, lipid production, and cellulose content in Chlorella vulgaris cultures using response surface methodology / Biotec
Berdy J / 2005 / Bioactive microbial metabolites / J. Antibiotics 58 : 1 ~ 26
Bogs J / 2000 / Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence / J. Bacteriol 182 : 5351 ~ 5358
Burke J / 2001 / Generalized transduction in Streptomyces coelicolor / Proc. Nat. Acad. Sci. USA 98 : 6289 ~ 6294
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