지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Streptomyces are widely distributed in soil and known for the production of bioactive secondary metabolites. It has been reported that among microbial-derived antibiotics, two-third are produced by the Streptomyces species alone. Hence, continuous screening of the Streptomyces species is of growing scientific interest. A small Himalayan country like Nepal is in a unique geographical location with a huge biodiversity. However, little is known about microbial diversity. The aim of this study was to isolate and characterize the Streptomyces species from a high-altitude soil sample collected from an altitude of 4,380 meters above sea level. The 16S rRNA sequence analysis revealed that four isolated strains; G-10, G-14, G-18, and S4L belong to the Streptomyces species. On the basis of phylogenetic analysis of 16S rRNA gene sequences of the isolates with the best match in the database revealed that G-18 isolates closely related to Streptomyces albidoflavus strain PAS-12. Moreover, the ranges of radical scavenging activities by crude extract of isolates were observed against DPPH and ABTS. Furthermore, crude extract of isolates revealed the range of antimicrobial activities against the four pathogenic strains namely Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus species, and Bacillus substilis. Moreover, isolated Streptomyces species revealed amylase, cellulase, and L-asparaginase enzyme activities.
목차
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (27)
참고문헌 신청
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Bull AT and Stach JE. 2007. Marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol. 15, 491–499.
Cheesman MJ, Ilanko A, Blonk B, and Cock IE. 2017. Developing new antimicrobial therapies: are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacogn. Rev. 11, 57–72.
Čihák M, Kameník Z, Šmídová K, Bergman N, Benada O, Kofroňová O, Petříčková K, and Bobek J. 2017. Secondary metabolites produced during the germination of Streptomyces coelicolor. Front. Microbiol. 8, 2495.
Dalisay DS, Williams DE, Wang XL, Centko R, Chen J, and Andersen RJ. 2013. Marine sediment-derived Streptomyces bacteria from British Columbia, Canada are a promising microbiota resource for the discovery of antimicrobial natural products. PLoS ONE 8, e77078.
Bull AT and Stach JE. 2007. Marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol. 15, 491–499.
Cheesman MJ, Ilanko A, Blonk B, and Cock IE. 2017. Developing new antimicrobial therapies: are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacogn. Rev. 11, 57–72.
Čihák M, Kameník Z, Šmídová K, Bergman N, Benada O, Kofroňová O, Petříčková K, and Bobek J. 2017. Secondary metabolites produced during the germination of Streptomyces coelicolor. Front. Microbiol. 8, 2495.
Dalisay DS, Williams DE, Wang XL, Centko R, Chen J, and Andersen RJ. 2013. Marine sediment-derived Streptomyces bacteria from British Columbia, Canada are a promising microbiota resource for the discovery of antimicrobial natural products. PLoS ONE 8, e77078.
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