지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Gliadin proteins are a major component of gluten proteins and important determinants of bread-making quality by conferring the viscosity and extensibility of dough, but also present significant health problems for consumers with wheat-related diseases like celiac disease or wheat allergies. In order to solve this problem, we conducted RP-HPLC analysis to profile gliadin fractions for screening the mutants deficient in gliadins from 122 wheat doubled-haploid (DH) lines cultivated by the National Institute of Crop Science. Comparing the RP-HPLC chromatogram of 122 DH lines with those of the respective parents, we found that some peaks of omega-5 gliadin were not present in 28 DH lines. Further analysis using SDS-PAGE and A-PAGE showed that the omega-5 gliadin in the parental varieties had two to three bands, but only one band in the absent 28 DH lines. The relative expression levels of all gliadin groups in the parental and mutant lines were also examined by RP-HPLC. Our study contributes to establishing a method for the rapid screening and identification of mutants missing gliadins as major epitopes of wheat-related disease in many wheat genetic resources and breeding lines as valuable information to other researchers.
목차
등록된 정보가 없습니다.
참고문헌 (58)
참고문헌 신청
Altenbach SB / 2014 / Quantitative proteomic analysis of wheat grain proteins reveals differential effects of silencing of omega-5 gliadin genes in transgenic lines / J Cereal Sci 59:118~125
Anderson OD / 2009 / The wheat ${\omega}$ -gliadin genes: structure and EST analysis / Funct Integr Genomics 9:397~410
Anderson OD / 2001 / The wheat ${\gamma}$ -gliadin genes: characterization of ten new sequences and further understanding of ${\gamma}$ -gliadin gene family structure / Theor Appl Genet 103:323~330
Anderson OD / 1997 / The ${\alpha}$ -gliadin gene family: 1. Characterization of ten new wheat ${\alpha}$ -gliadin genomic clones, evidence for limited sequence conservation of flanking DNA, and southern analysis of the gene family / Theor Appl Genet 95:50~58
Banc A / 2009 / Exploring the interactions of gliadins with model membranes: Effect of confined geometry and interfaces / Biopolym 91:610~622
Anderson OD / 2009 / The wheat
Anderson OD / 2001 / The wheat
Anderson OD / 1997 / The
Banc A / 2009 / Exploring the interactions of gliadins with model membranes: Effect of confined geometry and interfaces / Biopolym 91:610~622
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