지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Gliadin proteins, which are a component of gluten and confer viscosity and extensibility on wheat dough, are major determinants of wheat processing suitability and also present dietary problems for consumers with celiac disease or wheat allergies. In this study, gliadin proteins of the hexaploid wheat variety ‘Chinese Spring’ (CS) and of its nullisomic-tetrasomic (NT) and ditelosomic (DT) lines missing group 1 and 6 chromosome, were analyzed using LabChip GXII Touch 24 within 1 min per sample. The chromatogram pattern analysis of gliadin proteins from group 1 aneuploid lines (N1AT1B, N1AT1D; N1BT1A, N1BT1D; N1DT1A, N1DT1B) missing 1A, 1B and 1D chromosomes respectively, from CS showed that 24, 25 and 26 sec peaks of CS, presuming to be ω5-, ω1,2- and γ- gliadins, were disappeared. The analysis of group 6 aneuploid lines (N6AT6B, N6AT6D, 6AL; N6BT6A, 6BL; N6DT6B, 6DL) missing 6A, 6AS; 6B, 6BS; 6D, 6DS chromosomes respectively, from CS indicated that 22, 25 and 26 sec peaks of CS, presuming to be α-/β- gliadins, were disappeared. The results of this study will be applicable to high-throughput screening of wheat gliadin mutants among wheat breeding lines and genetic resources for the development of allergy - reduced wheat.
목차
등록된 정보가 없습니다.
참고문헌 (33)
참고문헌 신청
Banc A / 2009 / Exploring the interactions of gliadins with model membranes: Effect of confined geometry and interfaces / Biopolymers 91:610~622
Battais F / 2005 / Food allergy to wheat: differences in immunoglobin E-binding proteins as a function of age or symptoms / J Cereal Sci 42:109~117
Brown JWS / 1981 / Fractionation of wheat gliadin and glutenin subunits by two-dimensional electrophoresis and the role of group 6 and group 2 chromosomes in gliadins synthesis / Theor Appl Genet 59:349~359
Burns MA / 2002 / U.S. Patent No. 6,379,929 / U.S. Patent and Trademark Office
Cornish SL / 2006 / Formation of bright matter-wave solitons during the collapse of attractive Bose-Einstein condensates / Phys Rev Lett 96:170401
Battais F / 2005 / Food allergy to wheat: differences in immunoglobin E-binding proteins as a function of age or symptoms / J Cereal Sci 42:109~117
Brown JWS / 1981 / Fractionation of wheat gliadin and glutenin subunits by two-dimensional electrophoresis and the role of group 6 and group 2 chromosomes in gliadins synthesis / Theor Appl Genet 59:349~359
Burns MA / 2002 / U.S. Patent No. 6,379,929 / U.S. Patent and Trademark Office
Cornish SL / 2006 / Formation of bright matter-wave solitons during the collapse of attractive Bose-Einstein condensates / Phys Rev Lett 96:170401
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