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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제9호
- 발행연도
- 2017.1
- 수록면
- 1,566 - 1,575 (10page)
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초록· 키워드
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MicroRNAs (miRNAs) are abundant in bovine milk and milk derived from other livestock, and they have functional roles in infants and in the secretion process of mammary glands.
However, few studies have evaluated miRNAs in dairy processes, such as during cheese making and ripening. Thus, we investigated the characteristics of milk-derived miRNAs during the manufacturing and ripening of Camembert cheese as well as the microbiota present using the quantitative reverse transcription polymer chain reaction (RT-qPCR) and 16S rRNA pyrosequencing, respectively. Pyrosequencing showed that the cheese microbiota changed dramatically during cheese processing, including during the pasteurization, starter culture, and ripening stages. Our results indicated that the RNA contents per 200 mg/200 μl of the sample increased significantly during cheese-making and ripening. The inner cheese fractions had higher RNA contents than the surfaces after 12 and 22 days of ripening in a timedependent manner (21.9 and 13.2 times higher in the inner and surface fractions than raw milk, respectively). We performed a comparative analysis of the miRNAs in each fraction by RT-qPCR. Large amounts of miRNAs (miR-93, miR-106a, miR-130, miR-155, miR-181a, and miR- 223) correlated with immune responses and mammary glands were present in aged cheese, with the exception of miR-223, which was not present on the surface. Considerable amounts of miRNAs were also detected in whey, which is usually disposed of during the cheese-making process. Unexpectedly, there were no significant correlations between immune-related miRNAs and the microbial populations during cheese processing. Taken together, these results show that various functional miRNAs are present in cheese during its manufacture and that they are dramatically increased in amount in ripened Camembert cheese, with differences according to depth.
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참고문헌 (45)
참고문헌 신청
Haug A / 2007 / Bovine milk in human nutrition - a review / Lipids Health Dis 6:25
Koletzko B / 1998 / Growth, development and differentiation: a functional food science approach / Br. J. Nutr 80(Suppl 1):S5~S45
Chen X / 2010 / Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products / Cell Res 20:1128~1137
Hata T / 2010 / Isolation of bovine milk-derived microvesicles carrying mRNAs and microRNAs / Biochem. Biophys. Res. Commun 396:528~533
Schanzenbach CI / 2017 / Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle? / PLoS One 12:e0172220
Koletzko B / 1998 / Growth, development and differentiation: a functional food science approach / Br. J. Nutr 80(Suppl 1):S5~S45
Chen X / 2010 / Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products / Cell Res 20:1128~1137
Hata T / 2010 / Isolation of bovine milk-derived microvesicles carrying mRNAs and microRNAs / Biochem. Biophys. Res. Commun 396:528~533
Schanzenbach CI / 2017 / Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle? / PLoS One 12:e0172220
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