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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제4호
- 발행연도
- 2017.1
- 수록면
- 718 - 724 (7page)
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초록· 키워드
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The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the Cterminus of scFv57R in order to help it resist adverse stress and improve the stability and halflife. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.
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참고문헌 (24)
참고문헌 신청
Bakker A / 2008 / First administration to humans of a monoclonal antibody cocktail against rabies virus: safety, tolerability, and neutralizing activity / Vaccine 26 : 5922
Bird RE / 1988 / Single-chain antigen-binding proteins / Science 242 : 423 ~ 426
Bourhy H / 2010 / Rabies, still neglected after 125 years of vaccination / PLoS Negl. Trop. Dis 4 : e839 ~ e839
Cai K / 2008 / Novel human 3-domain disulfide-stabilized antibody fragment against glycoprotein of rabies virus / Microb. Infect 10 : 548 ~ 555
Cheng Y / 2015 / A VL-linker-VH orientation dependent single chain variable antibody fragment against rabies virus G protein with enhanced neutralizing potency in vivo / Prot
Bird RE / 1988 / Single-chain antigen-binding proteins / Science 242 : 423 ~ 426
Bourhy H / 2010 / Rabies, still neglected after 125 years of vaccination / PLoS Negl. Trop. Dis 4 : e839 ~ e839
Cai K / 2008 / Novel human 3-domain disulfide-stabilized antibody fragment against glycoprotein of rabies virus / Microb. Infect 10 : 548 ~ 555
Cheng Y / 2015 / A VL-linker-VH orientation dependent single chain variable antibody fragment against rabies virus G protein with enhanced neutralizing potency in vivo / Prot
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