지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 269 - 276 (8page)
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초록· 키워드
오류제보하기
Rabies virus (RABV)-specific antibodies in animals and humans are measured using standard methods such as fluorescent antibody virus neutralization (FAVN) tests and rapid fluorescent focus inhibition tests, which are based on cell culture systems. An alternative assay that is safe and easy to perform is required for rapid sero-surveillance following mass vaccination of animals. Two purified monoclonal antibodies (4G36 and B2H17) against RABV were selected as capture and detection antibodies, respectively. A genetically modified RABV, the ERAGS strain, was propagated and concentrated by polyethylene glycol precipitation. Optimal conditions for the RABV antigen, antibodies, and serum dilution for a blocking enzymelinked immune sorbent assay (B-ELISA) were established. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using serum samples from 138 dogs, 71 raccoon dogs, and 25 cats. The B-ELISA showed a diagnostic sensitivity of 95.8−96.3%, specificity of 91.3−100%, and accuracy of 96.0−97.2% compared to the FAVN test. These results suggest that the B-ELISA is useful for sero-surveillance of RABV in dogs, raccoon dogs, and cats.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Cheong Y / 2014 / Strategic model of national rabies control in Korea / Clin. Exp. Vaccine Res. 3:78~90
양동군 / 2017 / Mass vaccination has led to the elimination of rabies since 2014 in South Korea / Clinical and Experimental Vaccine Research 6(2):111~119
Cliquet F / 1998 / Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody / J. Immunol. Methods. 212:79~87
Fitzgerald E / 1996 / Potency test for antirabies serum and immunoglobulin / Laboratory Techniques in Rabies 4:417~422
Smith JS / 1973 / A rapid reproducible test for determining rabies neutralizing antibody / Laboratory Techniques in Rabies 4:181~192
양동군 / 2017 / Mass vaccination has led to the elimination of rabies since 2014 in South Korea / Clinical and Experimental Vaccine Research 6(2):111~119
Cliquet F / 1998 / Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody / J. Immunol. Methods. 212:79~87
Fitzgerald E / 1996 / Potency test for antirabies serum and immunoglobulin / Laboratory Techniques in Rabies 4:417~422
Smith JS / 1973 / A rapid reproducible test for determining rabies neutralizing antibody / Laboratory Techniques in Rabies 4:181~192
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