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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제4호
- 발행연도
- 2019.1
- 수록면
- 651 - 657 (7page)
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초록· 키워드
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Although smallpox was eradicated in 1980, it is still considered a potential agent of biowarfare and bioterrorism. Smallpox has the potential for high mortality rates along with a major public health impact, eventually causing public panic and social disruption. Passive administration of neutralizing monoclonal antibodies (mAbs) is an effective intervention for various adverse reactions caused by vaccination and the unpredictable nature of emerging and bioterroristrelated infections. Currently, vaccinia immune globulin (VIG) is manufactured from vaccinia vaccine-boosted plasma; however, this production method is not ideal because of its limited availability, low specific activity, and risk of contamination with blood-borne infectious agents. To overcome the limitations of VIG production from human plasma, we isolated two human single-chain variable fragments (scFvs), (SC34 and SC212), bound to vaccinia virus (VACV), from a scFv phage library constructed from the B cells of VACV vaccine-boosted volunteers. The scFvs were converted to human IgG1 (VC34 and VC212). These two anti- VACV mAbs were produced in Chinese Hamster Ovary (CHO) DG44 cells. The binding affinities of VC34 and VC212 were estimated by competition ELISA to IC50 values of 2 μg/ml (13.33 nM) and 22 μg/ml (146.67 nM), respectively. Only the VC212 mAb was proven to neutralize the VACV, as evidenced by the plaque reduction neutralization test (PRNT) result with a PRNT50 of ~0.16 mg/ml (~1.07 μM). This VC212 could serve as a valuable starting material for further development of VACV-neutralizing human immunoglobulin for a prophylactic measure against post-vaccination complications and for post-exposure treatment against smallpox.
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참고문헌 (18)
참고문헌 신청
Walsh SR / 2011 / Vaccinia viruses : vaccines against smallpox and vectors against infectious diseases and tumors / Expert. Rev. Vaccines 10:1221~1240
Moore ZS / 2006 / Smallpox / Lancet 367:425~435
Lustig S / 2009 / Effective post-exposure protection against lethal orthopoxviruses infection by vaccinia immune globulin involves induction of adaptive immune response / Vaccine 27:1691~1699
Wittek R / 2006 / Vaccinia immune globulin : current policies, preparedness, and product safety and efficacy / Int. J. Infect. Dis 10:193~201
Prescribing Information of CNJ-016, Vaccinia Immune Globulin Intravenous (Human), sterile solution / Emergent BioSolutions
Moore ZS / 2006 / Smallpox / Lancet 367:425~435
Lustig S / 2009 / Effective post-exposure protection against lethal orthopoxviruses infection by vaccinia immune globulin involves induction of adaptive immune response / Vaccine 27:1691~1699
Wittek R / 2006 / Vaccinia immune globulin : current policies, preparedness, and product safety and efficacy / Int. J. Infect. Dis 10:193~201
Prescribing Information of CNJ-016, Vaccinia Immune Globulin Intravenous (Human), sterile solution / Emergent BioSolutions
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