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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제5호
- 발행연도
- 2017.1
- 수록면
- 965 - 974 (10page)
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초록· 키워드
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The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, 55.2 ± 0.3ºC, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, 52.5 ± 0.3ºC, and 13.54 nm for the scFv expressed by E. coli.
This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.
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참고문헌 (22)
참고문헌 신청
Ahmad ZA / 2012 / scFv antibody: principles and clinical application / Clin. Dev. Immunol 2012 : 980250 ~
Andersen DC / 2004 / Production technologies for monoclonal antibodies and their fragments / Curr. Opin. Biotechnol 15 : 456 ~ 462
Babaei, Arash / 2011 / Production of a Recombinant Anti-Human CD4 Single-Chain Variable- Fragment Antibody Using Phage Display Technology and Its Expression in Escherichia c
Chen C / 2004 / High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain / Biotech
Cheng YM / 2015 / Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis / BMC Biotechnol 15 : 32 ~
Andersen DC / 2004 / Production technologies for monoclonal antibodies and their fragments / Curr. Opin. Biotechnol 15 : 456 ~ 462
Babaei, Arash / 2011 / Production of a Recombinant Anti-Human CD4 Single-Chain Variable- Fragment Antibody Using Phage Display Technology and Its Expression in Escherichia c
Chen C / 2004 / High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain / Biotech
Cheng YM / 2015 / Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis / BMC Biotechnol 15 : 32 ~
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