지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.9
- 수록면
- 148 - 155 (8page)
이용수
초록· 키워드
오류제보하기
Rabies is known as the most fatal disease in all warm-blooded animals, including dogs. Among animals that transmit rabies, dogs are mainly responsible for transmitting animal rabies in Asian countries. Detection of rabies virus (RABV) antibodies in dogs is performed by fluorescent antibody virus neutralization (FAVN) test or rapid fluorescent focus inhibition test. These standard assays are difficult to carry out in diagnostic laboratories without sufficient instruments, designated RABV, and cell culture systems. An alternative assay that is easy to conduct and time efficient is required for rapid sero-surveillance following vaccination. Recombinant baculovirus expressing RABV nucleoprotein (RVN) was constructed and the recombinant protein was purified using Ni-NTA and fast protein liquid column chromatography. We developed and evaluated an indirect enzyme-linked immunosorbent assay (I-ELISA) with recombinant RVN for the detection of RABV antibodies in 122 dog serum samples. The I-ELISA results obtained from these samples were compared with FAVN results. The sensitivity, specificity, and accuracy of I-ELISA were 88.1%, 92.5%, and 91.0%, respectively, compared with FAVN. Results of I-ELISA were significantly correlated with that of FAVN (r = 0.81). These results suggest that I-ELISA with recombinant RVN is useful for sero-surveillance of RABV in dog sera.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (17)
참고문헌 신청
Masatani T, Ito N, Shimizu K, Ito Y, Nakagawa K, Sawaki Y, et al. Rabies virus nucleoprotein functions to evade activation of the RIG-I-mediated antiviral response. J Virol 2010;84:4002-12.
Kuzmin IV, Hughes GJ, Rupprecht CE. Phylogenetic relationships of seven previously unclassified viruses within the family Rhabdoviridae using partial nucleoprotein gene sequences. J Gen Virol 2006;87:2323-31.
Katayama S, Yamanaka M, Ota S, Shimizu Y. A New quantitative method for rabies virus by detection of nucleoprotein in virion using ELISA. J Vet Med Sci 1999;61:411-6.
Yang DK, Kim HH, Lee KK, Yoo JY, Seomun H, Cho IS. Mass vaccination has led to the elimination of rabies since 2014 in South Korea. Clin Exp Vaccine Res 2017; 6:111-9.
Cheong Y, Kim B, Lee KJ, Park D, Kim S, Kim H, et al. Strategic model of national rabies control in Korea. Clin Exp Vaccine Res 2014;3:78-90.
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UCI(KEPA) : I410-ECN-0101-2018-475-001332036