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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 333 - 340 (8page)
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초록· 키워드
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An alkaline protease was purified and characterized from an alkalophilic microorganism, Bacillus sp. DK1122, isolated from soil in central Korea. The optimum temperature and pH for the growth of the producer strain were 40℃ and pH 9.0, respectively. The protease was produced aerobically at 40℃ after 24 h incubation in modified Horikoshi I medium (pH 9.0) containing 0.5% (w/v) glucose, 0.8% (w/v) yeast extract, 0.5% (w/v) polypeptone, 0.1% (w/v) K2HPO4, 0.02% (w/v) MgSO4·7H2O, 1% (w/v) Na2CO3, and 3% (w/v) NaCl. The alkaline protease was purified by 70% ammonium sulfate precipitation of the culture supernatant of Bacillus sp. DK1122, followed by CM-Sepharose chromatography. The molecular weight of the enzyme was estimated to be 27 kDa on the basis of SDS-PAGE. The optimum temperature and pH for the protease activity were 60℃ and pH 9.0, respectively. Addition of CaCl2 increased the thermal stability of the purified protease, where 90% of protease activity was retained at 60℃ for up to 3 h. Consequently, it is expected that the alkaline protease from this study, exhibiting stability at pH 7–9 and 60℃, may be promising for application in the food and detergent industries.
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참고문헌 (34)
참고문헌 신청
Ahn J-W / 1990 / Partial purification and characterization of the alkaline protease from Bacillus sp / Korean J. Appl. Microbiol. Biotechnol 18:344~351
Annamalai N / 2014 / Purification and characterization of solvent stable, alkaline protease from Bacillus firmus CAS 7 by microbial conversion of marine wastes and molecular mechanism underlying solvent stability / Process Biochem 49:1012~1019
방성호 / 2011 / 호알칼리성 Bacillus pseudofirmus HS-54가 생산하는 알칼리성 Protease의 특성 / 미생물학회지 47(3):194~199
Bayoudh A / 2000 / Purification and characterization of an alkaline protease from Pseudomonas aeruginosa MN1 / J. Ind. Microbial. Biotechnol 24:291~295
Beg QK / 2003 / Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis / Enzyme Microb. Technol 32:294~304
Annamalai N / 2014 / Purification and characterization of solvent stable, alkaline protease from Bacillus firmus CAS 7 by microbial conversion of marine wastes and molecular mechanism underlying solvent stability / Process Biochem 49:1012~1019
방성호 / 2011 / 호알칼리성 Bacillus pseudofirmus HS-54가 생산하는 알칼리성 Protease의 특성 / 미생물학회지 47(3):194~199
Bayoudh A / 2000 / Purification and characterization of an alkaline protease from Pseudomonas aeruginosa MN1 / J. Ind. Microbial. Biotechnol 24:291~295
Beg QK / 2003 / Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis / Enzyme Microb. Technol 32:294~304
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