지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제12호
- 발행연도
- 2017.1
- 수록면
- 2,190 - 2,198 (9page)
이용수
초록· 키워드
오류제보하기
Thermoactinomyces sp. strain YT06 was isolated from poultry compost and observed to degrade integral chicken feathers completely at 60°C, resulting in the formation of 3.24 mg/ml of free amino acids from 50 ml of culture containing 10 g/l chicken feathers. Strain YT06 could grow and secrete keratinase using feather as the only carbon and nitrogen sources without other supplement, but complementation of 10 g/l sucrose and 4 g/l NaNO3 increased the production of the keratinolytic enzyme. The maximum protease activity obtained was 110 U/ml and for keratinase was 42 U/ml. The keratinase maintained active status over a broad pH (pH 8-11) and temperature (60-75°C). It was inhibited by serine protease inhibitors and most metal ions; however, it could be stimulated by Mn2+ and the surfactant Tween-20. A reductive agent (β-mercaptoethanol) was observed to cleave the disulfide bond of keratin and improve the access of the enzyme to the keratinaceous substrate. Zymogram analysis showed that strain YT06 primarily secreted keratinase with a molecular mass of approximately 35 kDa. The active band was assessed by MALDI-TOF mass spectrometry and was observed to be completely identical to an alkaline serine protease from Thermoactinomyces sp. Gus2-1.
Thermoactinomyces sp. strain YT06 shows great potential as a novel candidate in enzymatic processing of hard-to-degrade proteins into high-value products, such as keratinous wastes.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Abdel-Naby MA / 2016 / Catalytic, kinetic and thermodynamic properties of Bacillus pumilus FH9 keratinase conjugated with activated pectin / Int. J. Biol. Macromol 85 : 238 ~
Brandelli A / 2010 / Biochemical features of microbial keratinases and their production and applications / Appl. Microbiol. Biotechnol 85 : 1735 ~ 1750
Błyskal B / 2009 / Fungi utilizing keratinous substrates / Int. Biodeterior. Biodegradation 63 : 631 ~ 653
Fang Z / 2014 / Cloning, heterologous expression and characterization of two keratinases from Stenotrophomonas maltophilia BBE11-1 / Process Biochem 49 : 647 ~ 654
Fellahi S / 2016 / Identification of two new keratinolytic proteases from a Bacillus pumilus strain using protein analysis and gene sequencing / AMB Express 6 : 1 ~ 8
Brandelli A / 2010 / Biochemical features of microbial keratinases and their production and applications / Appl. Microbiol. Biotechnol 85 : 1735 ~ 1750
Błyskal B / 2009 / Fungi utilizing keratinous substrates / Int. Biodeterior. Biodegradation 63 : 631 ~ 653
Fang Z / 2014 / Cloning, heterologous expression and characterization of two keratinases from Stenotrophomonas maltophilia BBE11-1 / Process Biochem 49 : 647 ~ 654
Fellahi S / 2016 / Identification of two new keratinolytic proteases from a Bacillus pumilus strain using protein analysis and gene sequencing / AMB Express 6 : 1 ~ 8
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Keratinase Production Using Non-food Source
한국미생물학회 학술대회논문집
2022 .10
-
호알칼리성 Bacillus sp. DK1122 균주가 생산하는 알칼리성 단백질 분해효소의 정제 및 특성
한국미생물·생명공학회지
2016 .01
-
Purification and Characterization of a Novel Extracellular Thermostable Alkaline Protease from Streptomyces sp. M30
Journal of Microbiology and Biotechnology
2015 .01
-
A Novel Thrombolytic and Anticoagulant Serine Protease from Polychaeta, Diopatra sugokai
Journal of Microbiology and Biotechnology
2018 .01
-
Identification of a Newly Isolated Protease-producing Bacterium, Bacillus subtilis FBL-1, from Soil
한국미생물·생명공학회지
2016 .01
-
Natural keratin extracted from chicken feathers as a gate dielectric material for organic thin film transistors
한국진공학회 학술발표회초록집
2016 .08
-
Janthinobacterium sp. 유래 저온 활성 프로테아제 정제
한국미생물·생명공학회지
2018 .01
-
반응표면분석법을 이용한 Bacillus amyloliquefaciens SRCM115785의 protease 활성증가를 위한 배지 최적화
생명과학회지
2021 .08
-
Exploring the Catalytic Significant Residues of Serine Protease Using Substrate-Enriched Residues and a Peptidase Inhibitor
한국미생물·생명공학회지
2021 .01
-
Optimization, Purification, and Characterization of Haloalkaline Serine Protease from a Haloalkaliphilic Archaeon Natrialba hulunbeirensis Strain WNHS14
한국미생물·생명공학회지
2021 .01
-
Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins
한국미생물·생명공학회지
2023 .06
-
Isolation of a Lipolytic and Proteolytic Bacillus licheniformis from Refinery Oily Sludge and Optimization of Culture Conditions for Production of the Enzymes
한국미생물·생명공학회지
2020 .01
-
Characterization of Flavobacterium sp. Strain I3-3 Isolated from River Water
한국미생물학회 학술대회논문집
2017 .04
-
Biodegradation of Feather Waste Keratin by the Keratin-Degrading Strain Bacillus subtilis 8
Journal of Microbiology and Biotechnology
2018 .01
-
Cultivable Microbial Diversity in Domestic Bentonites and Their Hydrolytic Enzyme Production
한국미생물·생명공학회지
2019 .01
-
Screening and Fermentation Characteristics of Bacillus sp. with High Amylase and Protease Activity Isolated from Traditional Nuruks
한국미생물학회 학술대회논문집
2016 .04
-
Characterization of Streptomyces sp. Strain JS029 as Potential Biological Control Agent against Broad Spectrum of Fungal Plant Pathogens
한국미생물학회 학술대회논문집
2021 .08
-
김치로부터 단백질 분해 효소활성이 우수한 Bacillus subtilis 균주의 분리
한국미생물·생명공학회지
2017 .01
-
저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석
한국해양바이오학회지
2019 .06
-
Crystallization and preliminary X-ray diffraction analysis of Thioredoxin from the feather-degrading thermophile Fervidobacterium islandicum AW-1
Biodesign
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0