지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 65 - 74 (10page)
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초록· 키워드
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Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.
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참고문헌 (35)
참고문헌 신청
Khan Z / 2019 / Bacillus tequilensis ZMS-2 : A novel source of alkaline protease with antimicrobial, anti-coagulant, fibrinolytic and dehairing potentials / Pak. J. Pharm. Sci 32:1913~1918
Bose A / 2014 / Production and characterization of a solvent-tolerant protease from a novel marine isolate Bacillus tequilensis P15 / Ann. Microbiol 64:343~354
Zaraî Jaouadi N / 2015 / A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process / Int. J. Biol. Macromol 79:952~964
Gupta R / 2002 / Bacterial alkaline proteases : molecular approaches and industrial applications / Appl. Microbiol. Biotechnol 59:15~32
Annamalai N / 2014 / Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes / Food Bioprod. Process 92:335~342
Bose A / 2014 / Production and characterization of a solvent-tolerant protease from a novel marine isolate Bacillus tequilensis P15 / Ann. Microbiol 64:343~354
Zaraî Jaouadi N / 2015 / A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process / Int. J. Biol. Macromol 79:952~964
Gupta R / 2002 / Bacterial alkaline proteases : molecular approaches and industrial applications / Appl. Microbiol. Biotechnol 59:15~32
Annamalai N / 2014 / Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes / Food Bioprod. Process 92:335~342
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