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Comparative genomics of the order Thermotogales revealed that the feather-degrading thermophilic eubacteriumFervidobacterium islandicum AW-1 has more extensive redox system comprising of enzymes thioredoxin (Trx) andthioredoxin reductase (TrxR) than non-feather degrading Fervidobacterium spp. Therefore, thioredoxin from F.islandicumAW-1 (FiTrx) likely plays a key role in the reduction of disulfide bridges present in feather keratin. In order to reveal theinsights of the electron transfer between feather keratin and thioredoxin systems in F.islandium AW-1, the preliminarystructural study of FiTrx was implemented. The FiTrx gene was cloned, expressed, purified, and crystallized. FiTrxcrystallized in 0.005M cobalt(II) chloride hexahydrate, 0.005 M nickel(II) chloride hexahydrate, 0.005 M cadmium chloridehydrate, 0.005 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5 and 12% w/v PEG3350, diffracted X-ray to a 1.25Å resolution and belonged to the space group P212121, with the unit cell parameters a = 35.81, b = 60.26, and c = 87.64 Å. The asymmetric unit of the crystal had two molecules with a calculated Mathew’s coefficient of 2.15 Å3 Da–1 and a solventcontent of 42.79%.
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참고문헌 (23)
참고문헌 신청
Adams, P. D. / 2002 / PHENIX : building new software for automated crystallographic structure determination / Acta Crystallogr D Biol Crystallogr 58:1948~1954
Arner, E. S. / 2000 / Physiological functions of thioredoxin and thioredoxin reductase / Eur J Biochem 267:6102~6109
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Friedrich, A. B. / 1996 / Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales / Appl Environ Microbiol 62:2875~2882
Headd, J. J. / 2012 / Use of knowledge-based restraints in phenix. refine to improve macromolecular refinement at low resolution / Acta Crystallogr D Biol Crystallogr 68:381~390
Arner, E. S. / 2000 / Physiological functions of thioredoxin and thioredoxin reductase / Eur J Biochem 267:6102~6109
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Friedrich, A. B. / 1996 / Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales / Appl Environ Microbiol 62:2875~2882
Headd, J. J. / 2012 / Use of knowledge-based restraints in phenix. refine to improve macromolecular refinement at low resolution / Acta Crystallogr D Biol Crystallogr 68:381~390
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