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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 185 - 193 (9page)
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A novel proteolytic bacterium was isolated from soil at Yeungnam University, South Korea. The strain, named FBL-1, was rod-shaped with a smooth surface. Biolog and API 50CHB test results revealed that strain FBL-1 was a Bacillus species. Based on 16S rDNA sequencing and chemotaxonomic characterization, the strain was identified as Bacillus subtilis because it had the highest homology with Bacillus subtilis subsp. subtilis NCIB 3610 (99.5%). In liquid culture at 37°C with shaking at 200 rpm, fructose and yeast extract were found to be the best carbon and nitrogen sources, respectively, for cell growth and protease production. The highest protease activity (451.640 U/ml) was obtained when the strain was cultured in medium containing 20 g/l of fructose and 5 g/l of yeast extract. Although further studies are needed to characterize the protease and enhance its activity, the newly isolated protein-degrading B. subtilis FBL-1 can be applicable for the production of peptides and for the degradation of proteins in various industries.
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참고문헌 (18)
참고문헌 신청
Adinarayana K / 2003 / Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11 / AAPS Pharm. Sci. Tech 4:1~9
Anissa H / 2010 / Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone / J. Biosci. Bioeng 110:288~294
Anson ML / 1938 / The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin / J. Gen. Physiol 22:79~89
Banerjee UC / 1999 / Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive / Process Biochem 35:213~219
Folin O / 1927 / On tyrosine and tryptophan determinations in proteins / J. Biol. Chem 73:627~650
Anissa H / 2010 / Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone / J. Biosci. Bioeng 110:288~294
Anson ML / 1938 / The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin / J. Gen. Physiol 22:79~89
Banerjee UC / 1999 / Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive / Process Biochem 35:213~219
Folin O / 1927 / On tyrosine and tryptophan determinations in proteins / J. Biol. Chem 73:627~650
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