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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제2호
- 발행연도
- 2018.1
- 수록면
- 314 - 322 (9page)
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Bacillus subtilis 8 is highly efficient at degrading feather keratin. We observed integrated feather degradation over the course of 48 h in basic culture medium while studying the entire process with scanning electron microscopy. Large amounts of ammonia, sulfite, and L-cysteic acid were detected in the fermented liquid. In addition, four enzymes (gammaglutamyltranspeptidase, peptidase T, serine protease, and cystathionine gamma-synthase) were identified that play an important role in this degradation pathway, all of which were verified with molecular cloning and prokaryotic expression. To the best of our knowledge, this report is the first to demonstrate that cystathionine gamma-synthase secreted by B. subtilis 8 is involved in the decomposition of feather keratin. This study provides new data characterizing the molecular mechanism of feather degradation by bacteria, as well as potential guidance for future industrial utilization of waste keratin.
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참고문헌 (28)
참고문헌 신청
Ramnani P / 2005 / Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation / Can. J. Microbiol. 51:191~196
Feng J / 1995 / Development and utilization of feather meal protein feed / Feed Ind. 1995:42~43
Gradisar H / 2005 / Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases / Appl. Environ. Microbiol. 71:3420~3426
Lopes FC / 2011 / Production of proteolytic enzymes by a keratin-degrading Aspergillus niger / Enzyme Res. 2011:487093
Huang Y / 2015 / Production and characterization of keratinolytic proteases produced by Onygena corvina / Fungal Genom. Biol. 4:119
Feng J / 1995 / Development and utilization of feather meal protein feed / Feed Ind. 1995:42~43
Gradisar H / 2005 / Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases / Appl. Environ. Microbiol. 71:3420~3426
Lopes FC / 2011 / Production of proteolytic enzymes by a keratin-degrading Aspergillus niger / Enzyme Res. 2011:487093
Huang Y / 2015 / Production and characterization of keratinolytic proteases produced by Onygena corvina / Fungal Genom. Biol. 4:119
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