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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제1호
- 발행연도
- 2015.1
- 수록면
- 66 - 73 (8page)
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초록· 키워드
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The pabS gene of Agaricus bisporus 02 encoding a putative PABA synthase was cloned, and then the recombinant protein was expressed in Escherichia coli BL21 under the control of the T7 promoter. The enzyme with an N-terminal GST tag or His tag, designated GST-AbADCS or His-AbADCS, was purified with glutathione Sepharose 4B or Ni Sepharose 6 Fast Flow. The enzyme was an aminodeoxychorismate synthase, and it was necessary to add with an aminodeoxychorismate lyase for synthesizing PABA. AbADCS has maximum activity at a temperature of approximately 25oC and pH 8.0. Magnesium or manganese ions were necessary for the enzymatic activity. The Michaelis-Menten constant for chorismate was 0.12 mM, and 2.55 mM for glutamine. H2O2 did distinct damage on the activity of the enzyme, which could be slightly recovered by Hsp20. Sulfydryl reagents could remarkably promote its activity, suggesting that cysteine residues are essential for catalytic function.
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참고문헌 (23)
참고문헌 신청
Basset G / 2002 / Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I / Proc. Natl. Acad. Sci. USA 99 : 124
Basset GJ / 2004 / Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase / Plant J 40 : 453 ~ 461
Basset GJ / 2004 / Folate synthesis in plants: the paminobenzoate branch is initiated by a bifunctional PabAPabB protein that is targeted to plastids / Proc. Natl. Acad. Sci.
Bera AK / 2012 / Structure of aminodeoxychorismate synthase from Stenotrophomonas maltophilia / Biochemistry 51 : 10208 ~ 10217
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Basset GJ / 2004 / Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase / Plant J 40 : 453 ~ 461
Basset GJ / 2004 / Folate synthesis in plants: the paminobenzoate branch is initiated by a bifunctional PabAPabB protein that is targeted to plastids / Proc. Natl. Acad. Sci.
Bera AK / 2012 / Structure of aminodeoxychorismate synthase from Stenotrophomonas maltophilia / Biochemistry 51 : 10208 ~ 10217
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
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