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자료유형
학술저널
저자정보
Kwon, Hyuk Woo (Department of Microbiology and Institute of Biodiversity, Dankook University) Choi, Min Ah (Department of Microbiology and Institute of Biodiversity, Dankook University) Yun, Yeo Hong (Department of Microbiology and Institute of Biodiversity, Dankook University) Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) Kim, Seong Hwan (Department of Microbiology and Institute of Biodiversity, Dankook University)
저널정보
한국균학회 Mycobiology Mycobiology 제43권 제1호
발행연도
2015.1
수록면
81 - 86 (6page)

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To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of IGS I, which ranged from 1,301 to 1,348 bp, were present among twenty ASI1346-derived progeny strains, while 15 different sizes of IGS I, which ranged from 700 to 1,347 bp, were present among twenty ASI1038-derived progeny strains. Phylogenetic analysis of the IGS sequences revealed that different clades were present in both the ASI10388- and ASI1346-derived progeny strains. Plating assays of seven kinds of extracellular enzymes (${\beta}$-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease) also revealed apparent variation in the ability to produce extracellular enzymes among the 40 tested progeny strains from both parental A. bisporus strains. Overall, this study demonstrates that characterization of IGS I regions and extracellular enzymes is useful for the assessment of the substrate-degrading ability and heterogenicity of A. bisporus monokaryotic strains.

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