지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 415 - 422 (8page)
이용수
초록· 키워드
오류제보하기
Terpenoids have a wide range of biological functions and have extensive applications in the pharmaceutical, cosmetic, and flavoring industry. The white-rot fungus, Polyporus brumalis, is able to synthesize terpenoids via terpene synthase, which catalyzes an important step that forms a large variety of sesquiterpene products from farnesyl pyrophosphate (FPP). To improve the production of sesquiterpenes, the terpene synthase gene was isolated from Polyporus brumalis and was heterologously transformed into a Pichia pastoris strain. The open reading frame of the isolated gene (approximately 1.2 kb) was inserted into Pichia pastoris to obtain a recombinant enzyme. Five transformants were obtained and the expression of terpene synthase was analyzed at the transcript level by reverse transcription PCR (polymerase chain reaction) and at the protein level by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Expression of the terpene synthase gene product was elevated in the transformants and as expected the molecular weight of the protein was approximately 45 kDa. These recombinant enzymes have potential practical applications and future studies should focus on their functional characterization.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Ahmad, M. / 2014 / Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production / Applied Microbiology and Biotechnology 98(12):5301~5317
Beibei, H. / 2008 / Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae / Biotechnology Letters 30(7):1121~1137
Bicas, J.L. / 2008 / Characterization of monoterpene biotransformation in two pseudomonads / Journal of Applied Microbiology 105(6):1991~2001
Cereghino, J.L. / 2000 / Heterologous protein expression in the methylotrophic yeast Pichia pastoris / FEMS Microbiology Reviews 24(1):45~66
Daly, R. / 2005 / Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production / Journal of Molecular Recognition 18(2):119~138
Beibei, H. / 2008 / Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae / Biotechnology Letters 30(7):1121~1137
Bicas, J.L. / 2008 / Characterization of monoterpene biotransformation in two pseudomonads / Journal of Applied Microbiology 105(6):1991~2001
Cereghino, J.L. / 2000 / Heterologous protein expression in the methylotrophic yeast Pichia pastoris / FEMS Microbiology Reviews 24(1):45~66
Daly, R. / 2005 / Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production / Journal of Molecular Recognition 18(2):119~138
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization and Differential Expression of Terpene Synthase and Farnesyl Pyrophosphate Synthase from Basidiomycetous Fungus Polyporus brumalis
한국미생물학회 학술대회논문집
2017 .04
-
Analyses of Terpene Synthase in a Tomato
한국미생물학회 학술대회논문집
2019 .04
-
Biosynthesis of Eudesmane-type Sesquiterpenoids by The Wood-rotting Fungus, Polyporus brumalis, on Specific Medium, including Inorganic Magnesium Source
목재공학(Journal of the Korean Wood Science and Technology)
2016 .01
-
Sesquiterpenoids Bioconversion Analysis by Wood Rot Fungi
한국균학회 학술대회
2016 .01
-
Exploring Novel Bacterial in the Family Fusibacter Terpene Synthases
한국미생물학회 학술대회논문집
2021 .08
-
Functional Production of Sialic Acid Binding Lectins Derived from Mushrooms in Pichia pastoris
한국미생물학회 학술대회논문집
2015 .04
-
Production, Purification, and Characterization of Phaseolus vulgaris Leghemoglobin a in Pichia pastoris
극지연구소 초록집
2023 .07
-
Analysis of Gene Expression Relating to Two Pathways on Terpene Backbone Biosynthesis
한국미생물학회 학술대회논문집
2015 .04
-
Profiling of major terpene in Cannabis species by headspace gas chromatography-mass spectrometry
한국분석과학회 학술대회
2022 .05
-
포도 과피에서 분리한 효모 Pichia manshurica와 Pichia terricola의 생리활성 증진효과
미생물학회지
2017 .12
-
Construction of Recombinant Pichia pastoris Carrying a Constitutive AvBD9 Gene and Analysis of Its Activity
Journal of Microbiology and Biotechnology
2015 .01
-
Improving 3’-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy
Journal of Microbiology and Biotechnology
2016 .01
-
Prediction and Purification of Sesquiterpene Synthase from Wood Rot Fungus
한국미생물학회 학술대회논문집
2016 .04
-
Development of Methods for the Separation of Major Cannabinoids and Terpenes using six Column Selectivity Screening
한국분석과학회 학술대회
2019 .11
-
Expression of a Tandemly Arrayed Plectasin Gene from Pseudoplectania nigrella in Pichia pastoris and its Antimicrobial Activity
Journal of Microbiology and Biotechnology
2016 .01
-
Heterologous Expression and Characterization of a Laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana
Journal of Microbiology and Biotechnology
2018 .01
-
Pichia pastoris에서강낭콩 Leghemoglobin a의생산, 정제및 특징
한국미생물·생명공학회지
2022 .12
-
Engineering of a Microbial Cell Factory for the Extracellular Production of Catalytically Active Phospholipase A2 of Streptomyces violaceoruber
Journal of Microbiology and Biotechnology
2020 .01
-
In Vitro N -Glycan Mannosyl-Phosphorylation of a Therapeutic Enzyme by Using Recombinant Mnn14 Produced from Pichia pastoris
Journal of Microbiology and Biotechnology
2021 .01
-
Small RNA Transcriptome of Hibiscus Syriacus Provides Insights into the Potential Influence of microRNAs in Flower Development and Terpene Synthesis
Molecules and Cells
2017 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0