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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 681 - 686 (6page)
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초록· 키워드
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The purpose of this study was to find a cold-adapted and surfactant-stable alginate lyase as a candidate for biotechnological and industrial applications. The gene for a new alginate lyase, AlyL1, from Agarivorans sp. L11 was cloned and expressed in Escherichia coli. The recombinant AlyL1 was most active at 40ºC (1,370 U/mg). It was a cold-adapted alginate lyase, which showed 54.5% and 72.1% of maximum activity at 15ºC and 20ºC, respectively. AlyL1 was an alkaliphilic enzyme and most active at pH 8.6. In addition, it showed high stability in the presence of various surfactants at a high concentration (from 0.1% to 1% (w/v)). AlyL1 was an endo-type alginate lyase that degraded both polyM and polyG blocks, yielding disaccharides and trisaccharides as the main products. This is the first report of the cloning and functional expression of a cold-adapted and surfactant-stable alginate lyase. AlyL1 might be an interesting candidate for biotechnological and industrial applications.
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참고문헌 (18)
참고문헌 신청
Falkeborga M / 2014 / Alginate oligosaccharides:enzymatic preparation and antioxidant property evaluation / Food Chem 164 : 185 ~ 194
Han F / 2004 / Cloning, sequence analysis and expression of gene alyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101 / DNA Seq 15 : 344 ~ 350
Iwamoto Y / 2003 / Enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells / Biosci. Biotechnol. Biochem 67 : 258 ~
Iwasaki K / 2000 / Purification of alginate oligosaccharides with root growth-promoting activity toward lettuce / Biosci. Biotechnol. Biochem 64 : 1067 ~ 1070
Kobayashi K / 2009 / A new high-alkaline alginate lyase from a deep-sea bacterium Agarivorans sp / Extremophiles 13 : 121 ~ 129
Han F / 2004 / Cloning, sequence analysis and expression of gene alyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101 / DNA Seq 15 : 344 ~ 350
Iwamoto Y / 2003 / Enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells / Biosci. Biotechnol. Biochem 67 : 258 ~
Iwasaki K / 2000 / Purification of alginate oligosaccharides with root growth-promoting activity toward lettuce / Biosci. Biotechnol. Biochem 64 : 1067 ~ 1070
Kobayashi K / 2009 / A new high-alkaline alginate lyase from a deep-sea bacterium Agarivorans sp / Extremophiles 13 : 121 ~ 129
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