지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제6호
- 발행연도
- 2010.1
- 수록면
- 1,011 - 1,017 (7page)
이용수
초록· 키워드
오류제보하기
A novel dioscin-glycosidase that specifically hydrolyzes multi-glycosides, such as 3-O-α-L-(1→4)-rhamnoside, 3-O-α-L-(1→2)-rhamnoside, 3-O-α-L-(1→4)-arabinoside, and β-D-glucoside, on diosgenin was isolated from the Absidia sp.d38 strain, purified, and characterized. The molecular mass of the new dioscin-glycosidase is about 55 kDa based on SDS-PAGE. The dioscin-glycosidase gradually hydrolyzes either 3-O-α-L-(1→4)-Rha or 3-O-α-L-(1→2)-Rha from dioscin into 3-O-α-L-Rha-β-D-Glc-diosgenin,further rapidly hydrolyzes the other α-L-Rha from 3-O-α-L-Rha-β-D-Glc-diosgenin into the main intermediate products of 3-O-β-D-Glc-diosgenin, and subsequently hydrolyzes these intermediate products into aglycone as the final product. The enzyme also gradually hydrolyzes 3-O-α-L-(1→4)-arabinoside, 3-O-α-L-(1→2)-rhamnoside, and β-Dglucoside from [3-O-α-L-(1→4)-Ara, 3-O-α-L-(1→2)-Rha]-β-D-Glc-diosgenin into diosgenin as the final product,exhibiting significant differences from previously reported glycosidases. The optimal temperature and pH for the new dioscin-glycosidase is 40oC and 5.0, respectively.
Whereas the activity of the new dioscin-glycosidase was not affected by Na+, K+, and Mg2+ ions, it was significantly inhibited by Cu2+ and Hg2+ ions, and slightly affected by Ca2+ ions.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Bae, E. A / 2000 / Constitutive β-glucosidases hydrolyzing ginsenoside Rb1 and Rb2 from human intestinal bacteria / Biol. Pharm. Bull 23 : 1481 ~ 1485
Bradford, M / 1976 / A rapid and sensitive method for quantization of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 : 203
Cai, J / 2002 / Apoptosis induced by dioscin in hela cells / Biol. Pharm. Bull 25 : 193 ~ 196
Feng, B / 2007 / Purification, characterization, and substrate specificity of a glucoamylase with steroidal saponinrhamnosidase activity from Curvularia lunata / Appl. Microb
Feng, B / 2007 / The substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata / Tetrahedron 63 : 6796 ~ 6812
Bradford, M / 1976 / A rapid and sensitive method for quantization of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 : 203
Cai, J / 2002 / Apoptosis induced by dioscin in hela cells / Biol. Pharm. Bull 25 : 193 ~ 196
Feng, B / 2007 / Purification, characterization, and substrate specificity of a glucoamylase with steroidal saponinrhamnosidase activity from Curvularia lunata / Appl. Microb
Feng, B / 2007 / The substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata / Tetrahedron 63 : 6796 ~ 6812
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Novel β-Glycosidase Belonging to Glycoside Hydrolase Family 30 from the Fungus Fomitopsis palustris
한국미생물학회 학술대회논문집
2017 .04
-
Cloning and Expression of Thermostable b-Glycosidase Gene from Thermusfiliformis Wai33 A1 in Escherichia coli and Enzyme Characterization
Journal of Microbiology and Biotechnology
2004 .01
-
Antifungal activity of steroidal saponins, dioscin and batatasin Ⅰ, isolated from root bark of Dioscorea nipponica and Dioscorea batatas
한국생명과학회 심포지움
2004 .10
-
Glycosidase Activity Profilie Using Coumarin-Conjugated Carbohydrate Derivatives
한국당과학회 학술대회
2006 .01
-
Crystal Structure of a Putative Alpha-glycosidase A from Thermotoga neapolitana
한국미생물학회 학술대회논문집
2011 .05
-
Mutational Analysis of Thermus caldophilus GK24 β-Glycosidase: Role ofHis119 in Substrate Binding and Enzyme Activity
Journal of Microbiology and Biotechnology
2008 .01
-
Composition Depth Profiling of Fe/Ni and Pt/Co Multilayers Using a Buckminsterfullerene (C60) Ion
한국진공학회 학술발표회초록집
2005 .08
-
Electron paramagnetic resonance spectra of natural spinel MgAl₂O₄ doped with Me³+ (Me=Cr³+, Fe³+) ions
한국자기학회 학술연구발표회 논문개요집
2003 .12
-
Enzymatic Characterization and Substrate Specificity of Thermostableβ-Glycosidase from Hyperthermophilic Archaea, Sulfolobus shibatae, Expressed in E. coli
Journal of Microbiology and Biotechnology
2007 .01
-
Thermostable β-Glycosidase-CBD Fusion Protein for Biochemical Analysisof Cotton Scouring Efficiency
Journal of Microbiology and Biotechnology
2008 .01
-
Modification of electronic properties of graphene by using low-energy K+ ions
한국진공학회 학술발표회초록집
2016 .08
-
Conformation of CRISPR-associated Csn2 DNA-binding Ring Is Regulated by Milimolar Concentration of Ca2+ Ion
한국미생물학회 학술대회논문집
2014 .04
-
Complete Biotransformation of Protopanaxatriol-Type Ginsenosides in Panax ginseng Leaf Extract to Aglycon Protopanaxatriol by β-Glycosidases from Dictyoglomus turgidum and Pyrococcus furiosus
Journal of Microbiology and Biotechnology
2018 .01
-
Enzymatic Synthesis of β-Glucosylglycerol and Its Unnatural Glycosides Via β-Glycosidase and Amylosucrase
Journal of Microbiology and Biotechnology
2019 .01
-
High density micro ion beam by plasma ion source
한국진공학회 학술발표회초록집
2016 .08
-
Fabrication of Potassium Ion Source and its Emission Characteristics
Applied Science and Convergence Technology
2016 .11
-
Linear Ion Beam Applications for Roll-to-Roll Metal Thin Film Coatings on PET Substrates
Applied Science and Convergence Technology
2015 .09
-
Na-ion batteries: from fundamental research to industrial exploration
한국진공학회 학술발표회초록집
2018 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0