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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제2호
- 발행연도
- 2018.1
- 수록면
- 255 - 261 (7page)
이용수
이 논문의 연구 히스토리 (2)
2018
Complete conversion of all typical glycosylated protopanaxatriol ginsenosides to aglycon protopanaxatriol by β-glycosidases from Dictyoglomus turgidum and Pyrococcus furiosus
양은주
생명공학과
2018.01
학위논문
Complete Biotransformation of Protopanaxatriol-Type Ginsenosides in Panax ginseng Leaf Extract to Aglycon Protopanaxatriol by β-Glycosidases from Dictyoglomus turgidum and Pyrococcus furiosus
양은주
,
신경철
,
이대영
외 1명
Journal of Microbiology and Biotechnology
2018.01
학술저널
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초록· 키워드
오류제보하기
Aglycon protopanaxatriol (APPT) has valuable pharmacological effects such as memory enhancement and tumor inhibition. β-Glycosidase from the hyperthermophilic bacterium Dictyoglomus turgidum (DT-bgl) hydrolyzes the glucose residues linked to APPT, but not other glycoside residues. β-Glycosidase from the hyperthermophilic bacterium Pyrococcus furiosus (PF-bgl) hydrolyzes the outer sugar at C-6 but not the inner glucose at C-6 or the glucose at C-20. Thus, the combined use of DT-bgl and PF-bgl is expected to increase the biotransformation of PPT-type ginsenosides to APPT. We optimized the ratio of PF-bgl to DTbgl, the concentrations of substrate and enzyme, and the reaction time to increase the biotransformation of ginsenoside Re and PPT-type ginsenosides in Panax ginseng leaf extract to APPT. DT-bgl combined with PF-bgl converted 1.0 mg/ml PPT-type ginsenosides in ginseng leaf extract to 0.58 mg/ml APPT without other ginsenosides, with a molar conversion of 100%. We achieved the complete biotransformation of ginsenoside Re and PPT-type ginsenosides in ginseng leaf extract to APPT by the combined use of two β-glycosidases, suggesting that discarded ginseng leaves can be used as a source of the valuable ginsenoside APPT. To the best of our knowledge, this is the first quantitative production of APPT using ginsenoside Re, and we report the highest concentration and productivity of APPT from ginseng extract to date.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Shin KC / 2016 / Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides / Crit. Rev. Biotechnol. 36:1036~1049
Xie JT / 2005 / Anti-diabetic effect of ginsenoside Re in ob/ob mice / Biochim. Biophys. Acta 1740:319~325
Cheng Y / 2005 / Anti-amnestic and antiaging effects of ginsenoside Rg1 and Rb1 and its mechanism of action / Acta Pharmacol. Sin. 26:143~149
김희진 / 2013 / A comprehensive review of the therapeutic and pharmacological effects of ginseng and ginsenosides in central nervous system / Journal of Ginseng Research 37(1):8~29
Naijing Li / 2016 / A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer’s disease / Journal of Ginseng Research 40(1):9~17
Xie JT / 2005 / Anti-diabetic effect of ginsenoside Re in ob/ob mice / Biochim. Biophys. Acta 1740:319~325
Cheng Y / 2005 / Anti-amnestic and antiaging effects of ginsenoside Rg1 and Rb1 and its mechanism of action / Acta Pharmacol. Sin. 26:143~149
김희진 / 2013 / A comprehensive review of the therapeutic and pharmacological effects of ginseng and ginsenosides in central nervous system / Journal of Ginseng Research 37(1):8~29
Naijing Li / 2016 / A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer’s disease / Journal of Ginseng Research 40(1):9~17
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Simultaneous Quantification of 13 Ginsenosides by LC-MS/MS and its Application in Diverse Ginseng Extracts
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동아시아식생활학회지
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Biotransformation of Protopanaxadiol- Type Ginsenosides in Korean Ginseng Extract into Food-Available Compound K by an Extracellular Enzyme from Aspergillus niger
Journal of Microbiology and Biotechnology
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Profiling of chemically transformed ginsenosides by steaming conditions of white ginseng based on UHPLC-Q/TOF-MS
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Changes of Ginsenoside Compositions in Cultivated Wild Ginseng with Different Steaming Temperatures and Time
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Tracing of chemical transformations of ginsenosides in white, red, and black ginsengs based on UHPLC-Q/TOF-MS
한국분석과학회 학술대회
2020 .11
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Development of HPLC-UV method for the detection and quantification of 30 ginsenoside in processed red ginseng products
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Study on Growth and Ginsenoside Content Changes in 5-6 Year Old GAP-Certified Ginseng from Geumsan
한국분석과학회 학술대회
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Quality analysis, ginsenosides and fragrance compounds confirmation of ginseng vinegar
한국분석과학회 학술대회
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Simultaneous determination of HPLC/PDA method for the detection and quantification of 33 ginsenosides in red ginseng
한국분석과학회 학술대회
2016 .11
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Induction of Ginsenosides Using Fungal Endophytes Isolated from Mountain-simulated Ginseng
한국미생물학회 학술대회논문집
2022 .10
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Quality control of ginseng and its products by determination of ginsenoside using LC-DAD-ELSD
한국분석과학회 학술대회
2016 .11
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Bacterial Diversity of a Single Soil Sample of a Ginseng Cultivating Field and Usage of Bacterial Enzymes from Isolates for Ginsenoside Bioconversion
한국미생물학회 학술대회논문집
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흰점박이꽃무지(Protaetia brevitarsis) 추출물의 항혈전 효능
한국환경과학회지
2019 .07
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Chryseobacterium panacisoli sp. nov., Isolated from Ginseng Cultivating Soil with Ginsenoside Converting Activity
한국미생물학회 학술대회논문집
2021 .08
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Ginsenosides analysis in the crude saponin fraction extracted from Korean red ginseng, and its efficacious analysis against acute pulmonary inflammation in mice
분석과학
2017 .06
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Inhibitory Effect of Ginsenosides Rh1 and Rg2 on Oxidative Stress in LPS-Stimulated RAW 264.7 Cells
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .12
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