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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제3호
- 발행연도
- 2008.1
- 수록면
- 443 - 448 (6page)
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초록· 키워드
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Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline cellulose (Avicel), the reporter protein is guided to the cellulose fibers that are increasingly exposed as the scouring process proceeds.
Among the tested reporter proteins, a thermostable β- glycosidase (BglA) from Thermus caldophilus was found to be most appropriate, showing a higher applicability and stability than GFP, DsRed2, or a tetrameric β-glucuronidase (GUS) from Escherichia coli, which were precipitated more seriously during the expression and purification steps.
When cotton fabrics with different scouring levels were treated with the BglA-CBD and incubated with X-Gal as the chromogenic substrate, an indigo color became visible within 2 h, and the color depth changed according to the conditions and extent of the scouring.
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참고문헌 (24)
참고문헌 신청
AATCC / / AATCC test method 39-1980. In: AATCC Test Manual. pp. 55: 286
CHUNG, HYE YOUNG / 2006 / Substitutions for Cys-472 and His-509 at the Active Site of β-Galactosidase from Lactococcus lactis ssp. lactis 7962 Cause Large Decreases in Enzym
Calafell, M. / 2004 / Effect of some process parameters in the enzymatic scouring cotton using an acid pectinase. Enzyme Microb / Technol 34 : 326 ~ 331
Carrard, G. / 2000 / Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl / Acad Sci 97 : 10342 ~ 10347
Choi, J. J. / 2003 / Enhanced expression of the gene for β-glycosidase of Thermus caldophilus GK24 and synthesis of galacto-oligosaccharides by the enzyme. Biotechnol. Appl
CHUNG, HYE YOUNG / 2006 / Substitutions for Cys-472 and His-509 at the Active Site of β-Galactosidase from Lactococcus lactis ssp. lactis 7962 Cause Large Decreases in Enzym
Calafell, M. / 2004 / Effect of some process parameters in the enzymatic scouring cotton using an acid pectinase. Enzyme Microb / Technol 34 : 326 ~ 331
Carrard, G. / 2000 / Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl / Acad Sci 97 : 10342 ~ 10347
Choi, J. J. / 2003 / Enhanced expression of the gene for β-glycosidase of Thermus caldophilus GK24 and synthesis of galacto-oligosaccharides by the enzyme. Biotechnol. Appl
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