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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제6호
- 발행연도
- 2010.1
- 수록면
- 995 - 1,000 (6page)
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초록· 키워드
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A novel deoC gene was identified from Paenibacillus sp.
EA001 isolated from soil. The gene had an open reading frame (ORF) of 663 base pairs encoding a protein of 220 amino acids with a molecular mass of 24.5 kDa.
The amino acid sequence was 79% identical to that of deoxyribose 5-phosphate aldolase (DERA) from Geobacillus sp. Y412MC10. The deoC gene encoding DERA was cloned into an expression vector and the protein was expressed in Escherichia coli. The recombinant DERA was purified using Ni-NTA affinity chromatography and then characterized. The optimum temperature and pH of the enzyme were 50oC and 6.0, respectively. The specific activity for the substrate deoxyribose 5-phosphate (DR5P)was 62 μmol/min/mg. The Km value for DR5P was determined to be 145 mM with the kcat value of 3.2×102/s from Lineweaver-Burk plots. The EA001 DERA showed stability toward a high concentration of acetaldehyde (100 mM).
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참고문헌 (19)
참고문헌 신청
Barbas III, C. F / 1990 / Deoxyribose-5-phosphate aldolase as a synthetic catalyst / J. Am. Chem. Soc 112 : 2013 ~ 2014
DeSantis, G / 2003 / Structure-based mutagenesis approaches toward expanding the substrate specificity of D-2-deoxyribose-5-phosphate aldolase / Bioorg. Med. Chem 11 : 43 ~ 5
Feron, G / 2007 / Microbial production of 4-hydroxybenzylidine acetone, the direct precursor of raspberry ketone / Lett. Appl. Microbiol 45 : 29 ~ 35
Greenberg, W. A / 2004 / Development of an efficient, scalable, aldolase-catalyzed process for enantioselective synthesis of statin intermediates / Proc. Natl. Acad. Sci. U.S.A : 5
Han, T. K / 2004 / Identification, molecular cloning, and sequence analysis of a deoxyribose aldolase in Streptococcus mutans GS-5 / Curr. Microbiol 48 : 230 ~ 236
DeSantis, G / 2003 / Structure-based mutagenesis approaches toward expanding the substrate specificity of D-2-deoxyribose-5-phosphate aldolase / Bioorg. Med. Chem 11 : 43 ~ 5
Feron, G / 2007 / Microbial production of 4-hydroxybenzylidine acetone, the direct precursor of raspberry ketone / Lett. Appl. Microbiol 45 : 29 ~ 35
Greenberg, W. A / 2004 / Development of an efficient, scalable, aldolase-catalyzed process for enantioselective synthesis of statin intermediates / Proc. Natl. Acad. Sci. U.S.A : 5
Han, T. K / 2004 / Identification, molecular cloning, and sequence analysis of a deoxyribose aldolase in Streptococcus mutans GS-5 / Curr. Microbiol 48 : 230 ~ 236
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