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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제8호
- 발행연도
- 2011.1
- 수록면
- 861 - 868 (8page)
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초록· 키워드
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A xylanase gene, xyn7c, was cloned from Paenibacillus sp.
12-11, an alkalophilic strain isolated from the alkaline wastewater sludge of a paper mill, and expressed in Escherichia coli. The full-length gene consists of 1,296 bp and encodes a mature protein of 400 residues (excluding the putative signal peptide) that belongs to the glycoside hydrolase family 10. The optimal pH of the purified recombinant XYN7C was found to be 8.0, and the enzyme had good pH adaptability at 6.5-8.5 and stability over a broad pH range of 5.0-11.0. XYN7C exhibited maximum activity at 55℃ and was thermostable at 50℃ and below.
Using wheat arabinoxylan as the substrate, XYN7C had a high specific activity of 1,886 U/mg, and the apparent K_m and V_(max) values were 1.18 mg/ml and 1,961 μmol/mg/min,respectively. XYN7C also had substrate specificity towards various xylans, and was highly resistant to neutral proteases. The main hydrolysis products of xylans were xylose and xylobiose. These properties make XYN7C a promising candidate to be used in biobleaching, baking,and cotton scouring processes.
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참고문헌 (22)
참고문헌 신청
Bajpai, P / 1999 / Application of enzymes in the pulp and paper industry / Biotechnol. Prog 15 : 147 ~ 157
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications: A review / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Caballero, P. A. / 2007 / Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination / J. Food Process Eng 81 : 42 ~ 53
Christov, L. P / 1999 / Production, partial characterization and use of fungal cellulase-free xylanases in pulp bleaching / Process Biochem 34 : 511 ~ 517
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications: A review / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Caballero, P. A. / 2007 / Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination / J. Food Process Eng 81 : 42 ~ 53
Christov, L. P / 1999 / Production, partial characterization and use of fungal cellulase-free xylanases in pulp bleaching / Process Biochem 34 : 511 ~ 517
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