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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제11호
- 발행연도
- 2014.1
- 수록면
- 1,525 - 1,535 (11page)
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The xynC gene, which encodes high molecular weight xylanase from Paenibacillus sp. DG-22, was cloned and expressed in Escherichia coli, and its nucleotide sequence was determined. The xynC gene comprised a 4,419bp open reading frame encoding 1,472 amino acid residues, including a 27 amino acid signal sequence. Sequence analysis indicated that XynC is a multidomain enzyme composed of two family 4_9 carbohydrate-binding modules (CBMs), a catalytic domain of family 10 glycosyl hydrolases, a family 9 CBM, and three S-layer homologous domains. Recombinant XynC was purified to homogeneity by heat treatment, followed by Avicel affinity chromatography. SDS-PAGE and zymogram analysis of the purified enzyme identified three active truncated xylanase species. Protein sequencing of these truncated proteins showed that all had identical N-terminal sequences. In the protein characterization, recombinant XynC exhibited optimal activity at pH 6.5 and 65oC and remained stable at neutral to alkaline pH (pH 6.0-10.0). The xylanase activity of recombinant XynC was strongly inhibited by 1 mM Cu2+ and Hg2+, whereas it was noticeably enhanced by 10 mM dithiothreitol. The enzyme exhibited strong activity towards xylans, including beechwood xylan and arabinoxylan, whereas it showed no cellulase activity. The hydrolyzed product patterns of birchwood xylan and xylooligosaccharides by thin-layer chromatography confirmed XynC as an endoxylanase.
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참고문헌 (24)
참고문헌 신청
[학술지(정
Ali MK / 2001 / Importance of the carbohydrate-binding module of Clostridium stercorarium Xyn10B to xylan hydrolysis / Biosci. Biotechnol. Biochem 65 : 41 ~ 47
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST:a new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
Bataillon M / 2000 / Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 / Enzyme Microb. Technol 26 : 187 ~ 192
Beg QK / 2001 / Microbial xylanases and their industrial applications: a review / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
Ali MK / 2001 / Importance of the carbohydrate-binding module of Clostridium stercorarium Xyn10B to xylan hydrolysis / Biosci. Biotechnol. Biochem 65 : 41 ~ 47
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST:a new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
Bataillon M / 2000 / Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 / Enzyme Microb. Technol 26 : 187 ~ 192
Beg QK / 2001 / Microbial xylanases and their industrial applications: a review / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
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