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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제7호
- 발행연도
- 2009.1
- 수록면
- 709 - 712 (4page)
이용수
초록· 키워드
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Enzymatic glucosylation with glycosyltransferases can be
used to regulate the water solubility of aglycone. The
drawback of this process is the demand of UDP-glucose as
a sugar donor. We made an in-frame fusion of the
flavonoid O-glucosyltransferase (OsUGT-3) and sucrose
synthase (AtSUS) genes. The resulting fusion protein,
OsUGT3-AtSUS, was expressed in E. coli and purified.
When sucrose and UDP were supplied, the fusion protein
was able to convert quercetin into quercetin O-glucoside
without the addition of UDP-glucose. In addition, UDPglucose
was recycled when sucrose was added to the
reaction mixture. This fusion protein is useful for the
enzymatic production of flavonoid O-glucosides.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Boudet, A.-M. / 2007 / Evolution and current status of research in phenolic compounds / Phytochemistry 68 : 2722 ~ 2735
Bowles, D. / 2005 / Glycosyltransferases: Managers of small molecules / Curr. Opin. Plant Biol 8 : 254 ~ 263
Breton, C. / 1999 / Structure/function studies of glycosyltransferases / Curr. Opin. Struct. Biol 9 : 563 ~ 571
Espin, J. C. / 2007 / Nutraceuticals: Facts and fiction / Phytochemistry 68 : 2986 ~ 3008
Gachon, C. M. M. / 2005 / Plant secondary metabolism glycosyltransferases: The emerging functional analysis / Trends Plant Sci 10 : 542 ~ 549
Bowles, D. / 2005 / Glycosyltransferases: Managers of small molecules / Curr. Opin. Plant Biol 8 : 254 ~ 263
Breton, C. / 1999 / Structure/function studies of glycosyltransferases / Curr. Opin. Struct. Biol 9 : 563 ~ 571
Espin, J. C. / 2007 / Nutraceuticals: Facts and fiction / Phytochemistry 68 : 2986 ~ 3008
Gachon, C. M. M. / 2005 / Plant secondary metabolism glycosyltransferases: The emerging functional analysis / Trends Plant Sci 10 : 542 ~ 549
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