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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제4호
- 발행연도
- 2007.1
- 수록면
- 571 - 578 (8page)
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A monoclonal antibody (mab) against theantimicrobial sulfamethazine was prepared and characterizedby an indirect competitive enzyme-linked immunosorbentassay (IC-ELISA). Sulfamethazine in the range of 0.2 and45 ng/ml could be determined with the mab by IC-ELISA.cDNAs encoding a variable heavy chain and variable light chainof the mab were cloned to produce recombinant antibodiesusing phage display technology. Following phage rescue andthree rounds of panning, a single-chain variable fragment(scFv) antibody with high sulfamethazine-binding affinitywas obtained. ELISA analysis revealed that scFv antibody andparent mab showed similar, but not identical, characteristics.The IC50 value by IC-ELISA with scFv antibody was 4.8 ng/ml,compared with 1.6 ng/ml with the parent mab. Performancesthe mab-based assay was less affected than the scFv-basedassay. Sixty milk samples were analyzed by mab-based IC-ELISA, and four samples were sulfamethazine positive; theseresults were favorably corelated with those obtained byHPLC.
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참고문헌 (24)
참고문헌 신청
Anna. Y. K / 2003 / Fluorescence polarization immunoassay based on a monoclonal antibody for the detection of the organophosphorus pesticide parathion-methyl 51 : 1107 ~ 1114
C / / and G. M. Rogister. 1993. Determination of sulfamethazine in animal tissues by enzyme immunoassay. Anal. Chim. Acta 275 : 323 ~ 328
Choi, J. Y / 2002 / Identification of calmodulin isoform-specific binding peptides from a phage-displayed random 22-mer peptide library 277 : 21630 ~ 21638
Fodey, T. L / 1997 / Comparison of porcine urine and bile as matrices to screen for the residues of two sulfonamides using a semi-automated enzyme immunoassay 122 : 165 ~ 168
Fuh, M. R. S. / 2001 / Quantitative determination of sulfonamide in meat by liquid chromatographyelectrospray- mass spectrometry 55 : 1127 ~ 1139
C / / and G. M. Rogister. 1993. Determination of sulfamethazine in animal tissues by enzyme immunoassay. Anal. Chim. Acta 275 : 323 ~ 328
Choi, J. Y / 2002 / Identification of calmodulin isoform-specific binding peptides from a phage-displayed random 22-mer peptide library 277 : 21630 ~ 21638
Fodey, T. L / 1997 / Comparison of porcine urine and bile as matrices to screen for the residues of two sulfonamides using a semi-automated enzyme immunoassay 122 : 165 ~ 168
Fuh, M. R. S. / 2001 / Quantitative determination of sulfonamide in meat by liquid chromatographyelectrospray- mass spectrometry 55 : 1127 ~ 1139
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