지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 52 - 59 (8page)
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Epidermal growth factor receptor (EGFR) is an attractive target for tumor therapy because it is overexpressed in the majority of solid tumors and the increase in receptor expression levels has been linked with a poor clinical prognosis. Also it is well established that blocking the interaction of EGFR and the growth factors could lead to the arrest of tumor growth and possibly result in tumor cell death. A13 is a murine monoclonal antibody (mAb) that specifically binds to various sets of EGFR-expressing tumor cells and inhibits EGF-induced EGFR phosphorylation. We isolated human immunoglobulin genes by guided selection based on the mAb A13. Four different human single chain Fvs (scFvs) were isolated from from hybrid scFv libraries containing a human VH repertoire with the VL of mAb A13 and a human VL repertoire with the VH of mAb A13. All the 4 scFvs bound to EGFR-expressing A431 cells. One scFv (SC414) with the highest affinity was converted to IgG1 (ER414). The ER414 exhibited ∼17 fold lower affinity compared to the A13 mAb. In addition the ER414 inhibited an EGF-induced tyrosine phosphorylation of EGFR with much lower efficacy compared to the A13 mAb and Cetuximab (Merck KgaA, Germany). We identified that the epitope of A13mAb is retained in ER414. This approach will provide an efficient way of converting a murine mAb to a human mAb.
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참고문헌 (23)
참고문헌 신청
Bao GQ / 2005 / Isolating human antibody against human hepatocellular carcinoma by guided-selection / Cancer Biol Ther 4 : 1374 ~ 1380
Baselga J / 2002 / Targeting the epidermal growth factor receptor with tyrosine kinase inhibitors: small molecules, big hopes / J Clin Oncol 20 : 2217 ~ 2219
Beiboer SH / 2000 / Guided selection of a pan carcinoma specific antibody reveals similar binding characteristics yet structural divergence between the original murine antibo
Brabender J / 2001 / Epidermal growth factor receptor and HER2-neu mRNA expression in non-small cell lung cancer Is correlated with survival / Clin Cancer Res 7 : 1850 ~ 1855
Capdevila J / 2009 / Anti-epidermal growth factor receptor monoclonal antibodies in cancer treatment / Cancer Treat Rev 35 : 354 ~ 363
Baselga J / 2002 / Targeting the epidermal growth factor receptor with tyrosine kinase inhibitors: small molecules, big hopes / J Clin Oncol 20 : 2217 ~ 2219
Beiboer SH / 2000 / Guided selection of a pan carcinoma specific antibody reveals similar binding characteristics yet structural divergence between the original murine antibo
Brabender J / 2001 / Epidermal growth factor receptor and HER2-neu mRNA expression in non-small cell lung cancer Is correlated with survival / Clin Cancer Res 7 : 1850 ~ 1855
Capdevila J / 2009 / Anti-epidermal growth factor receptor monoclonal antibodies in cancer treatment / Cancer Treat Rev 35 : 354 ~ 363
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