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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제5호
- 발행연도
- 2018.1
- 수록면
- 776 - 783 (8page)
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초록· 키워드
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The agarase gene gaa16a was identified from a draft genome sequence of Gilvimarinus agarilyticus JEA5, an agar-utilizing marine bacterium. Recently, three agarase-producing bacteria, G. chinensis, G. polysaccharolyticus, and G. agarilyticus, in the genus Gilvimarinus were reported. However, there have been no reports of the molecular characteristics and biochemical properties of these agarases. In this study, we analyzed the molecular characteristics and biochemical properties of agarases in Gilvimarinus. Gaa16A comprised a 1,323-bp open reading frame encoding 441 amino acids. The predicted molecular mass and isoelectric point were 49 kDa and 4.9, respectively. The amino acid sequence of Gaa16A showed features typical of glycosyl hydrolase family 16 (GH16) β-agarases, including a GH16 domain, carbohydrate-binding region (RICIN domain), and signal peptide. Recombinant Gaa16A (excluding the signal peptide and carbohydrate-binding region, rGaa16A) was expressed as a fused protein with maltose-binding protein at its N-terminus in Escherichia coli. rGaa16A had maximum activity at 55°C and pH 7.0 and 103 U/mg of specific activity in the presence of 2.5 mM CaCl2. The enzyme hydrolyzed agarose to yield neoagarotetraose as the main product. This enzyme may be useful for industrial production of functional neoagaro-oligosaccharides.
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참고문헌 (46)
참고문헌 신청
Chi WJ / 2012 / Agar degradation by microorganisms and agar-degrading enzymes / Appl. Microbiol. Biotechnol. 94:917~930
Fu XT / 2010 / Agarase: review of major sources, categories, purification method, enzyme characteristics and applications / Mar. Drugs 8:200~218
Araki C / 1956 / Structure of the agarose constituent of agar- agar / Bull. Chem. Soc. Jpn. 29:543~544
Mai Z / 2016 / Isolation and characterization of a glycosyl hydrolase family 16 beta-agarase from a mangrove soil metagenomic library / Int. J. Mol. Sci. 17:E1360
Kobayashi R / 1997 / Neoagarobiose as a novel moisturizer with whitening effect / Biosci. Biotechnol. Biochem. 61:162~163
Fu XT / 2010 / Agarase: review of major sources, categories, purification method, enzyme characteristics and applications / Mar. Drugs 8:200~218
Araki C / 1956 / Structure of the agarose constituent of agar- agar / Bull. Chem. Soc. Jpn. 29:543~544
Mai Z / 2016 / Isolation and characterization of a glycosyl hydrolase family 16 beta-agarase from a mangrove soil metagenomic library / Int. J. Mol. Sci. 17:E1360
Kobayashi R / 1997 / Neoagarobiose as a novel moisturizer with whitening effect / Biosci. Biotechnol. Biochem. 61:162~163
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