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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제12호
- 발행연도
- 2009.1
- 수록면
- 1,514 - 1,519 (6page)
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초록· 키워드
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A gene encoding the xylanase of Bacillus subtilis AMX-4
isolated from soil was cloned into Escherichia coli and the
gene product was purified from the cell-free extract of the
recombinant strain. The gene, designated xylA, consisted of
639 nucleotides encoding a polypeptide of 213 residues. The
deduced amino acid sequence was highly homologous to
those of xylanases belonging to glycosyl hydrolase family
11. The molecular mass of the purified xylanase was
23 kDa as estimated by SDS-PAGE. The enzyme had a
pH optimum of 6.0-7.0 and a temperature optimum of
50-55oC. Xylanase activity was significantly inhibited by
5 mM Cu2+ and 5 mM Mn2+, and noticeably enhanced by
5 mM Fe2+. The enzyme was active on xylans including
arabinoxylan, birchwood xylan, and oat spelt xylan, but it
did not exhibit activity toward carboxymethylcellulose or
p-nitrophenyl-β-xylopyranoside. The predominant products
resulting from xylan and xylooligosaccharide hydrolysis
were xylobiose and xylotriose. The enzyme could hydrolyze
xylooligosaccharides larger than xylotriose.
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참고문헌 (22)
참고문헌 신청
[학술지
Balakrishnan, H. / 2002 / Characterization of alkaline thermoactive cellulase-free xylanases from alkalophilic Bacillus (NCL 87-6-10) / J. Biochem. Mol. Biol. Biophys 6 : 325
Bataillon, M. / 2000 / Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 / Enzyme Microb. Technol 26 : 187 ~ 192
Bernier, R. Jr. / 1983 / Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli / Gene 26 : 59 ~ 65
Blanco, A. / 1995 / Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23 / Appl. Environ. Microbiol 61 : 4468 ~ 4470
Balakrishnan, H. / 2002 / Characterization of alkaline thermoactive cellulase-free xylanases from alkalophilic Bacillus (NCL 87-6-10) / J. Biochem. Mol. Biol. Biophys 6 : 325
Bataillon, M. / 2000 / Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 / Enzyme Microb. Technol 26 : 187 ~ 192
Bernier, R. Jr. / 1983 / Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli / Gene 26 : 59 ~ 65
Blanco, A. / 1995 / Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23 / Appl. Environ. Microbiol 61 : 4468 ~ 4470
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