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An alkaliphilic bacterium, Bacillus sp. strain BK,was found to produce extracellular cellulase-free xylanolyticenzymes with xylan-binding activity. Since the pellet-boundxylanase is eluted with 2% TEA from the pellet of the culture,they contain a xylan-binding region that is stronger than thexylan-binding xylanase of the extracelular enzyme. Thexylanases had a diferent molecular weight and xylan-bindingability. The enzyme activity of xylanase in the extracellularAmong the enzymes, xylanase had the highest enzymeactivity. When Bacillus sp. strain BK was grown in pH 10.5alkaline medium containing xylan as the sole carbon source,the bacterium produced xylanase, arabinofuranosidase, acetylesterase, and β-xylosidase with specific activities of 1.23,0.1, 0.06, and 0.04 unit per mg of protein, respectively.However, there was no cellulase activity detected in the crudeenzyme preparation. The hydrolysis of agricultural residues50oC and pH 7.0. The rate of xylan hydrolysis in corn hullwas higher than those of sugarcane bagasse, rice straw, corncop, rice husk, and rice bran. In contrast, the rate of xylanhydrolysis in sugarcane pulp was 2.01 and 3.52 times higherthan those of eucalyptus and pine pulp, respectively. Inconclusion, this enzyme can be used to hydrolyze xylan inagricultural residues and kraft pulps to breach and regeneratepaper from recycled environmental resources.

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