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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제3호
- 발행연도
- 2008.1
- 수록면
- 404 - 409 (6page)
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초록· 키워드
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The brown-rot basidiomycete Fomitopsis palustris is known to degrade crystalline cellulose (Avicel) and produce three major cellulases, exoglucanases, endoglucanases, and β- glucosidases. A gene encoding endoglucanase, designated as cel12, was cloned from total RNA prepared from F.
palustris grown at the expense of Avicel. The gene encoding Cel12 has an open reading frame of 732 bp, encoding a putative protein of 244 amino acid residues with a putative signal peptide residing at the first 18 amino acid residues of the N-terminus of the protein. Sequence analysis of Cel12 identified three consensus regions, which are highly conserved among fungal cellulases belonging to GH family 12. However, a cellulose-binding domain was not found in Cel12, like other GH family 12 fungal cellulases.
Northern blot analysis showed a dramatic increase of cel12 mRNA levels in F. palustris cells cultivated on Avicel from the early to late stages of growth and the maintenance of a high level of expression in the late stage, suggesting that Cel12 takes a significant part in endoglucanase activity throughout the growth of F. palustris. Adventitious expression of cel12 in the yeast Pichia pastoris successfully produced the recombinant protein that exhibited endoglucanase activity with carboxymethyl cellulose, but not with crystalline cellulose, suggesting that the enzyme is not a processive endoglucanase unlike two other endoglucanases previously identified in F. palustris.
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참고문헌 (23)
참고문헌 신청
Biely, P. / 1985 / Sensitive detection of endo-1,4-β-glucanases and endo-1,4-β-xylanases in gels. Anal / Biochem 144 : 147 ~ 151
Bourne, Y. / 2001 / Glycoside hydrolases and glycosyltransferases families and functional modules. Curr. Opin. Struct / Biol 11 : 593 ~ 600
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 72 :
Cohen, R. / 2005 / Processive endoglucanase active in crystalline cellulose hydrolysis by the brown rot basidiomycete Gloeophyllum trabeum. Appl. Environ / Microbiol 71 : 241
Gilkes, N. R. / 1991 / Domains in microbial beta-1,4-glycanases: Sequence conservation, function, and enzyme families. Microbiol / Rev 55 : 303 ~ 315
Bourne, Y. / 2001 / Glycoside hydrolases and glycosyltransferases families and functional modules. Curr. Opin. Struct / Biol 11 : 593 ~ 600
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 72 :
Cohen, R. / 2005 / Processive endoglucanase active in crystalline cellulose hydrolysis by the brown rot basidiomycete Gloeophyllum trabeum. Appl. Environ / Microbiol 71 : 241
Gilkes, N. R. / 1991 / Domains in microbial beta-1,4-glycanases: Sequence conservation, function, and enzyme families. Microbiol / Rev 55 : 303 ~ 315
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