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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 160 - 166 (7page)
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A DNA fragment containing 2,079 base pairs from Bacillus
circulans CGMCC 1416 was cloned using degenerate
PCR and inverse PCR. An open reading frame containing
981 bp was identified that encoding 326 amino acids
residues, including a putative signal peptide of 31 residues.
The deduced amino acid sequence showed the highest
identity (68.1%) with endo-β-1,4-D-mannanase from Bacillus
circulans strain K-1 of the glycoside hydrolase family 5
(GH5). The sequence encoding the mature protein was cloned
into the pET-22b(+) vector and expressed in Escherichia coli
as a recombinant fusion protein containing an N-terminal
hexahistidine sequence. The fusion protein was purified
by Ni2+ affinity chromatography and its hexahistidine
tag cleaved to yield a 31-kDa β-mannanase having a
specific activity of 481.55 U/mg. The optimal activity of
the purified protein, MANB48, was at 58oC and pH 7.6.
The hydrolysis product on substrate locust bean gum
included a monosaccharide and mainly oligosaccharides.
The recombinant MANB48 may be of potential use in the
feed industry.
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참고문헌 (21)
참고문헌 신청
Araujo, A. / 1990 / Hemicellulases of Bacillus species: Preliminary comparative studies on production and properties of mannanases and galactanases. J. Appl / Bacteriol 68 :
Arcand, D. N. / 1993 / β-Mannanase of Streptomyces lividans 66: Cloning and DNA sequence of the manA gene and characterization of the enzyme / Biochem 290 : 857 ~ 863
Baird, D. S. / 1990 / The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo- β-1,4-glucanases is essential for enzymatic activity. Biochem. Biop
Belaich, A. / 1992 / The catalytic domain of endoglucanase A from Clostridium cellulolyticum: Effects of arginine 79 and histidine 122 mutations on catalysis / Bacteriol 174
Bewley, D. J. / 1997 / Molecular cloning of a cDNA encoding a (1-4)-β-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum) / Planta 203 : 454 ~
Arcand, D. N. / 1993 / β-Mannanase of Streptomyces lividans 66: Cloning and DNA sequence of the manA gene and characterization of the enzyme / Biochem 290 : 857 ~ 863
Baird, D. S. / 1990 / The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo- β-1,4-glucanases is essential for enzymatic activity. Biochem. Biop
Belaich, A. / 1992 / The catalytic domain of endoglucanase A from Clostridium cellulolyticum: Effects of arginine 79 and histidine 122 mutations on catalysis / Bacteriol 174
Bewley, D. J. / 1997 / Molecular cloning of a cDNA encoding a (1-4)-β-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum) / Planta 203 : 454 ~
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