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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 322 - 328 (7page)
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Fermented skate is a traditional Korean food popular in Southwestern area of Korea. It has a characteristic flavor and alkaline pH. In this study we tried to determine the microbial flora in fermented skate using two different approaches. In culture-independent method, we amplified V2 region of 16S rRNA gene by PCR and cloned them into pUC18 plasmid to construct 16S rDNA fragment library. BLAST searches for the sequences obtained from this library revealed that uncultured bacterium clone 054E11.b was the most dominant flora in this fermented fish. In culture-dependent method, we diluted suspension of skate and spreaded on MRS, PCA, and MacConkey plates. We identified colonies grown on those plates by using PCR amplification of V2 region of 16S rRNA and DNA sequencing. BLAST searches of those DNA sequences resulted in totally different species with the observations from the 16S rDNA library analysis. Discrepancies of results obtained from both approaches suggest that the agar plates used in culture-dependent method may be different from the real condition of fermented skate. Therefore, results from culture-independent approach using 16S rDNA fragment library analysis may reflect real microbial flora in fermented skate.
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참고문헌 (16)
참고문헌 신청
Amann, R. I / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev. 59 : 143 ~ 169
Ercolini,D / 2004 / PCR-DGGE fingerprinting: novel strategies for detection of microbes in food / J. Microbiol. Methods 56 : 297 ~ 314
Felske, A. L / 1998 / Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophores
Ferris, M. J / 1996 / Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community / Appl. Environ. Microb
Kim, S. Y / 2007 / Biological and genetic classification of canine intestinal lactic acid bacteria and bifidobacteria / Microbiol. Immunol. 51 : 919 ~ 928
Ercolini,D / 2004 / PCR-DGGE fingerprinting: novel strategies for detection of microbes in food / J. Microbiol. Methods 56 : 297 ~ 314
Felske, A. L / 1998 / Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophores
Ferris, M. J / 1996 / Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community / Appl. Environ. Microb
Kim, S. Y / 2007 / Biological and genetic classification of canine intestinal lactic acid bacteria and bifidobacteria / Microbiol. Immunol. 51 : 919 ~ 928
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