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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 412 - 418 (7page)
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초록· 키워드
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To develop a novel therapeutic angiogenesis for the treatment of cardiovascular diseases, angiogenin (ANG1) was examined as a potential therapeutic gene. An adeno-associated virus (AAV)-mediated gene delivery system was used to measure the therapeutic efficacy of ANG1. Using a triple cotransfection technique, rAAV-ANG1-GFP, rAAVVEGF- GFP and rAAV-GFP vectors were produced, which were then used to infect human umbilical vein endothelial cells (HUVECs) in order to evaluate in vitro angiogenic activities. Their protein expres - sions, tagged with green fluorescent protein (GFP),were monitored by confocal microscopy. The functional activities were measured using woundhealing HUVEC migration assays. The number of migrated cells stimulated by both the expressed ANG1 and the VEGF in rAAV-infected HUVECs increased almost twice the number observed in the expressed GFP control. In vivo angiogenic activities of the expressed ANG1 or VEGF were determined using mouse angiogenesis assays. The angiogenic activities of ANG1 or VEGF expressed in the injected mice were increased by 1.36 and 2.16 times, respectively, compared to those of the expressed GFP control. These results demonstrate that the ex - pressed ANG1 derived from rAAV infection has in vitro and in vivo angiogenic activities and suggest that the rAAV-ANG1 vector is a potential strategy for therapeutic angiogenesis.
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참고문헌 (16)
참고문헌 신청
Celletti FL / 2001 / Vascular endothelial growth factor enhances atherosclerotic plaque progression 7 : 425 ~ 429
Herzog RW / 1997 / Stable gene transfer and expression of human blood coagulation factor IX after intramuscular injection of recombinant adeno-associated virus 94 : 5804 ~ 58
Hsieh MY / 2005 / AAV-mediated gene transfer to endothelial cells 11 : S153 ~ S154
Kastrup J / 2003 / Therapeutic angiogenesis in ischemic heart disease gene or recombinant vascular growth factor protein therapy? 3 : 197 ~ 206
Kim HJ / 2004 / Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease 36 : 336 ~ 344
Herzog RW / 1997 / Stable gene transfer and expression of human blood coagulation factor IX after intramuscular injection of recombinant adeno-associated virus 94 : 5804 ~ 58
Hsieh MY / 2005 / AAV-mediated gene transfer to endothelial cells 11 : S153 ~ S154
Kastrup J / 2003 / Therapeutic angiogenesis in ischemic heart disease gene or recombinant vascular growth factor protein therapy? 3 : 197 ~ 206
Kim HJ / 2004 / Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease 36 : 336 ~ 344
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