지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 48 - 51 (4page)
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Purpose: To assess whether the expression of heat shock protein 72 (Hsp72) protects rat retinal ganglion cells (RGC-5) from apoptotic cell death.
Methods: Hsp72 expression in RGC-5 cells transduced with replication-deficient recombinant adenovirus was analyzed by Western blot analysis and immunofluorescence. The effect of Hsp72 expression on etoposide-induced apoptotic cell death was examined by microscopic analysis and confirmed by cell proliferation assay.
Results: Western blot analysis and immunofluorescence clearly showed adenovirus-mediated Hsp72 expression in RGC-5 cells. Treatment with etoposide resulted in the death of a proportion of the cells by apoptosis. However, this apoptotic cell death was significantly reduced in cells expressing Hsp72, with the reduction in cell death correlating to the level of Hsp72 expression.
Conclusions: Over-expression of Hsp72 alone is sufficient to rescue neuronal cells from apoptotic cell death, suggesting that fine-tuning its expression may be an effective neuroprotective approach in retinal degenerative disease
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참고문헌 (20)
참고문헌 신청
Amin V / 1996 / Over-expression of heat shock protein 70 protects neuronal cells against both thermal and ischaemic stress but with different efficiencies / Neurosci Lett 206
Asea A / 2000 / HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine / Nat Med 6 : 435 ~ 442
Barbe MF / 1988 / Hyperthermia protects against light damage in the rat retina / Science 241 : 1817 ~ 1820
Beere HM. / 2004 / “The stress of dying”: the role of heat shock proteins in the regulation of apoptosis / J Cell Sci 117 (13) : 2641 ~ 2651
Calderwood SK / 2007 / Extracellular heat shock proteins in cell signaling and immunity / Ann N Y Acad Sci 1113 : 28 ~ 39
Asea A / 2000 / HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine / Nat Med 6 : 435 ~ 442
Barbe MF / 1988 / Hyperthermia protects against light damage in the rat retina / Science 241 : 1817 ~ 1820
Beere HM. / 2004 / “The stress of dying”: the role of heat shock proteins in the regulation of apoptosis / J Cell Sci 117 (13) : 2641 ~ 2651
Calderwood SK / 2007 / Extracellular heat shock proteins in cell signaling and immunity / Ann N Y Acad Sci 1113 : 28 ~ 39
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