분리배양된 신생 흰쥐 나선신경절세포의 면역염색학적 특징

정원호; 홍성화; 노형일; 김명순; 장병찬; 김창훈

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자료유형: 학술저널

저자정보: 정원호 홍성화 노형일 김명순 장병찬 김창훈

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제45권 제5호

발행연도: 2002.1

수록면: 433 - 438 (6page)

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Background and Objectives：Neuronal dissociation culture is an important tol for the study of neuronal cel survival and ganglion neurons in vivo. This study was aimed to establish the culture systems for the spinal neuronal cells and to characterize the cultured cells using different neuronal marker. Materials and Methods：Dissociated spiral ganglion cells were harvested from Sprague Dawley rats in postnatal 5 or 6 days and cultured for 48 hours. To prepare in vivo section, the harvested cochlea was embedded in parafin and sectioned in 4 μm thickness. Both cultured cells and parafin sections were labeled with seve-ral monoclonal antibodies (NSE, NF-200, NF-160, S-10) unoflourescent staining methods. Results：Each of the antibodies was used to stain both cultured cells and parafin sections. NSE was used to stain the nuclei of neuronal cells. Either NS or NR was used to stain both neuronal perikarya and neurite. The Schwan cells were stained by S-100. There was no significant difference in the immunostaining pattern between cochlea tissue and dissociated cells of spiral ganglion. Conclusion：A dissociated culture system for the spiral ganglions was established. In system can be used for the study of neuronal cell biology. (Korean J Otolaryngol 2002;45:433-8)

#나선신경절·분리배양. #Spiral ganglion· Dissociation.

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정원호

소속기관 Department of Otorhinolaryngology-Head and Neck Surgery, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Korea

주요연구분야 의약학 > 임상의학 > 이비인후과학 의약학 > 임상의학 > 신경과학

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소속기관 성균관대학교