배양된 와우신경절에서 분화된 신경세포에서의신경접착분자와 Polysialic Acid의 발현

박경호; Helge Rask-Andersen; Frederic A. Troy II; 박시내; 배민영; 조상재; 이흥구; 김준규; 손동화; 여상원

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자료유형: 학술저널

저자정보: 박경호 Helge Rask-Andersen Frederic A. Troy II 박시내 배민영 조상재 이흥구 김준규 손동화 여상원

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제50권 제1호

발행연도: 2007.1

수록면: 31 - 36 (6page)

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Background and Objectives：Neural cell adhesion molecule (NCAM) and polysialic acid (PSA) function basically in cell adhesion and migration. In neural development, they are closely asociated with axon pathfinding, synaptogenesis, neural cell migration, differentiation and myelination. The purpose of this study is to ases expresion of NCAM and PSA expresion in spiral ganglion neurons and Schwann cells and to postulate their functions. Materials and Method：Guinea pig spiral ganglion cells were harvested and cultured in vitro. The cels were grown and differentiated in culture medium together with brain derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) (GDNF). After 1 wek of culturing, the cells were fixed and immunocytochemical staining with -III tubulin, S-100, polysialic acid (PSA) and neural cell adhesion molecule (NCAM) were performed. We then checked axon growth rate with Axon Analyzer System. Results：In the spiral ganglion culture, cultured neurons showed positive staining for -III tubulin, NCAM, and different expresions of PSA. S-100 positive glial cells (Schwann cells) showed diferent expresions of NCAM and no expression of PSA. Some NCAM positive ne-- 30 μm/h using Axon Analyzer System. Conclusion：We postulated that NCAM may play an important role in neural cell adhesion, myelination, fasciculation and ganglion formation. But PSA did not expres the adhesive function of NCAM；its absence may have ben due to developmental reason. The diferential expresion of NCAM in the Schwann cells may indicate its diferent imunocytochemical characteristics and functions as shown in the CNS glial cells, astrocytes and oligodendrocytes. (Korean J Otolaryngol 2007 ;50 :31-6)

#와우신경절 신경원세포·신경교세포·Poly -sialic acid·신경접착분자. #Spiral ganglion neuron· Schwann cel· Polysialic acid· Neural cell adhesion molecule.

참고문헌 (16)

참고문헌 신청

/ / From bacteria to brains. Glycobiology 1992 : 5 ~ 23

/ / Roth J. Polysialic acid is aso-ciated with sodium channels and the neural cell adhesion molecule NCAM in adult rat brain. J Biol Chem 1992 : 9965 ~ 71

/ / Sunshine J. The ne-ural cell adhesion molecule as a regulator of cell-cell interactions. Science 1988 : 53 ~ 7

/ / im-munoglobulin like domains : 799 ~ 806

/ 1842-9. / Vimr ER. Specific al-teration of NCAM mediated cell adhesion by an endoneuraminidase. J Cell Biol 1985 : 5 ~

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