지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 98 - 105 (8page)
이용수
초록· 키워드
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Objectives The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated.
Materials and Methods One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 104, 2.5 × 104, and 5.0 × 104. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility).
Results Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 104 hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01).
Conclusions The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
de Chevigny C / 2008 / Treatment outcome in endodontics: the Toronto study-phase 4: initial treatment / J Endod 34:258~263
Ricucci D / 2011 / A prospective cohort study of endodontic treatments of 1,369 root canals: results after 5 years / Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112:825~842
Nirmalanandhan VS / 2006 / Effects of cell seeding density and collagen concentration on contraction kinetics of mesenchymal stem cell-seeded collagen constructs / Tissue Eng 12:1865~1872
Nirmalanandhan VS / 2007 / Effect of length of the engineered tendon construct on its structure-function relationships in culture / J Biomech 40:2523~2529
Chandrahasa S / 2011 / Proliferation of mature ex vivo human dental pulp using tissue engineering scaffolds / J Endod 37:1236~1239
Ricucci D / 2011 / A prospective cohort study of endodontic treatments of 1,369 root canals: results after 5 years / Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112:825~842
Nirmalanandhan VS / 2006 / Effects of cell seeding density and collagen concentration on contraction kinetics of mesenchymal stem cell-seeded collagen constructs / Tissue Eng 12:1865~1872
Nirmalanandhan VS / 2007 / Effect of length of the engineered tendon construct on its structure-function relationships in culture / J Biomech 40:2523~2529
Chandrahasa S / 2011 / Proliferation of mature ex vivo human dental pulp using tissue engineering scaffolds / J Endod 37:1236~1239
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