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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 53 - 61 (9page)
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Purpose: The chemokine receptor CXCR4 plays a role
in the metastasis and progression of a broad range of
malignant tumors; however, its influence on hepatocellular
carcinoma (HCC) is not well defined. Thus,
we analyzed the expression of CXCR4 and its functions
in HCC cell lines in vitro.
Materials and Methods: Five HCC cell lines (HepG2,
Hep3B, SK-HEP-1, NCI-H630 and PLC/PRF5) were investigated.
The CXCR4 expression was analyzed by
RT-PCR, Western blotting, flow cytometry and immunofluorescence
staining. In addition, the effects of stromal
cell-derived factor-1 (SDF-1) on the migration, proliferation
and survival of the cells were investigated, as well as
the SDF-1-induced phosphorylation of signaling molecules.
Results: All five cell lines had abundant CXCR4 in their
cytoplasm, whereas a cell surface CXCR4 expression
was only detected in a very small population of PLC/
PRF5 cells. In contrast, SDF-1 bound to all the cells. SDF-1
induced the phosphorylation of AKT and ERK1/2 in the
PLC/PRF5 cells and the phosphorylation of Stat3, AKT
and ERK1/2 in the Hep3B cells. Nonetheless, SDF-1 did
not induce migration or proliferation in any of the cells, nor
did it rescue the cells from serum deprivation-induced
apoptosis. Recruitment of CXCR4 from the cytoplasm to
the cell surface was not elicited by dexamethasone,
proinflammatory cytokines or VEGF. Hypoxia increased
both the cytoplasmic and cell surface expressions of
CXCR4 in only the PLC/PRF5 cells.
Conclusions: CXCR4 is trapped in the cytoplasm and it
is not recruited to the cell surface by standard extrinsic
stimuli in the majority of HCC cell lines, and the result of
this is a negligible response to SDF-1.
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참고문헌 (21)
참고문헌 신청
Altenburg JD / 2007 / A naturally occurring splice variant of CXCL12/ stromal cell-derived factor 1 is a potent human immunodeficiency virus type 1 inhibitor with weak chemot
Balabanian K. / 2005 / The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor RDC1 in T lymphocytes / J Biol Chem 280 : 35760 ~ 6
Begum NA / 1999 / Reduced expression of the CXCR4 receptor mRNA in hepatocellular carcinoma and lack of inducibility of its ligand alpha-chemokine hIRH/SDF1alpha/PBSF in vit
Bruhl H. / 2003 / Post-translational and cell type-specific regulation of CXCR4 expression by cytokines / Eur J Immunol 33 : 3028 ~ 37
Burns JM / 2006 / novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development / J Exp Med 203 : 2201 ~ 13
Balabanian K. / 2005 / The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor RDC1 in T lymphocytes / J Biol Chem 280 : 35760 ~ 6
Begum NA / 1999 / Reduced expression of the CXCR4 receptor mRNA in hepatocellular carcinoma and lack of inducibility of its ligand alpha-chemokine hIRH/SDF1alpha/PBSF in vit
Bruhl H. / 2003 / Post-translational and cell type-specific regulation of CXCR4 expression by cytokines / Eur J Immunol 33 : 3028 ~ 37
Burns JM / 2006 / novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development / J Exp Med 203 : 2201 ~ 13
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