지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 57 - 65 (9page)
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Objectives: Bone marrow-derived mesenchymal stem cells (BMSCs) are a good source for cartilage repair. Chondral defects do not heal spontaneously due to a lack of chondrocytes at the defect site and migration of surrounding chondrocytes to the injury site. The purpose of this study was to determine the expression of 19 chemokine receptors in human BMSCs stimulated by IL-1β and TNF-α as an in vitro cartilage-injury condition.
Materials & Methods: MSCs isolated from human bone marrow were expended for the experiments. Specifically, cells were grown either in the presence or absence of pro-inflammatory cytokines, such as IL-1β and TNF-α. RT-PCR was performed to evaluate the expression of 19 chemokine receptors. To evaluate changes in the expression of chemokine receptors, a reverse dot-blot assay was performed.
Results: We observed increased expression of CCR4, CCR6, CCR7, CCR9, CCR10, CXCR1, CXCR4, CXCR6, and CXCR7 in untreated BMSCs, while inflammation-induced BMSCs exhibited enhanced expression of CCR9, CCR10, CXCR1, CXCR4, CXCR5, CXCR6, and CXCR7. Collectively, the results showed CCR9/ CYA-25, CCR10/SCYA-28, CXCR1/IL-8, CXCR4/SDF-1, CXCR6/CXCL-16, and CXCR7/SDF-1 (receptor/ligand) were expressed in normal BMSCs and showed a gradual increase upon treatment with pro-inflammatory cytokines.
Conclusions: We suggest that chemokines, including SCYA-25, SCYA-28, IL-8, CXCL-16 and SDF-1 can be useful in repairing damaged articular cartilage.
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참고문헌 (25)
참고문헌 신청
Baggiolini M / 2001 / Chemokines in pathology and medicine / J Intern Med 250 : 91 ~ 104
Brittberg M / 1994 / Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation / N Engl J Med 331 : 889 ~ 895
Buckwalter JA / 1995 / Osteoarthritis and articular cartilage use, disuse and abuse: Experimental studies / J Rheumatol 22 : 13 ~ 15
Buckwalter JA / 1998 / Articular cartilage repair and transplantation / Arthritis Rheum 41 : 1331 ~ 1342
Buckwalter JA / 2000 / Synovial joint degeneration and the syndrome of
Brittberg M / 1994 / Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation / N Engl J Med 331 : 889 ~ 895
Buckwalter JA / 1995 / Osteoarthritis and articular cartilage use, disuse and abuse: Experimental studies / J Rheumatol 22 : 13 ~ 15
Buckwalter JA / 1998 / Articular cartilage repair and transplantation / Arthritis Rheum 41 : 1331 ~ 1342
Buckwalter JA / 2000 / Synovial joint degeneration and the syndrome of
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