Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

Hye Young Na; Moah Sohn; Seul Hye Ryu; Wanho Choi; Hyunju In; Hyun Soo Shin; Chae Gyu Park

Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

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Type: Academic journal

Author: Hye Young Na (Yonsei University College of Medicine) Moah Sohn (Yonsei University College of Medicine) Seul Hye Ryu (Yonsei University College of Medicine) Wanho Choi (Yonsei University College of Medicine) Hyunju In (Yonsei University College of Medicine) Hyun Soo Shin (Yonsei University College of Medicine)

Journal: The Korean Association of Immunologists Immune Network Vol.18 No.2 KCI Accredited Journals SCIE

Published: 2018.4

Pages: 68 - 79 (12page)

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Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

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Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naïve T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner. We found that, except for programmed death-ligand 1, most cell surface molecules were expressed lower in the BM cells cultured with GM-CSF for the extended duration. These results indicate that BM cells in the extended culture with GM-CSF undergo 2 distinct steps of functional change; first, they lose the immunostimulatory capacity; and next, they gain the immunosuppressive ability.

#Bone marrow #Dendritic cells #Granulocyte-macrophage colony-stimulating factor #Lymphocyte culture test, mixed #Immunosuppression

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Steinman RM / 1978 / Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice / Proc Natl Acad Sci U S A 75:5132~5136

Steinman RM / 2012 / Decisions about dendritic cells: past, present, and future / Annu Rev Immunol 30:1~22

Park CG / 2014 / Vaccine strategies utilizing C-type lectin receptors on dendritic cells in vivo / Clin Exp Vaccine Res 3:149~154

Fogg DK / 2006 / A clonogenic bone marrow progenitor specific for macrophages and dendritic cells / Science 311:83~87

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